摘要
目的:探讨不同组别(据精液参数分组)精子在不同时间段的前向运动精子百分率(PR)、总活动率(PR+NP)和顶体酶活性的变化率。方法:根据WHO《人类精液检查与处理实验室手册》第5版的判定标准和改良巴氏染色将精液分为3组。精子活化后,采用CASA检测在3个时间点(1、24、48 h)的PR和PR+NP,改良Kennedy法检测顶体酶活性。结果:1~24 h段与24~48 h段比较,3组精子的PR和PR+NP的变化均有显著性差异(P〈0.05),前段的变化小于后段;而顶体酶在正常组有显著性差异(P=0.013),前段的变化大于后段,在弱畸精子症组与少弱畸精子症组中的变化均无差异(P=0.519和0.979)。结论:活化后精子的PR、PR+NP和顶体酶活性均随时间的延长而降低,3个指标的变化率有不同的规律。应将顶体酶活性推广列入男性生育能力评估的常规检测项目。
Objective: To investigate the progressive motility, (PR), total motility (progressive + non-progressive motility, PR + NP), and acrosin activity of sperm from normal and infertile men at different time points after sperm activation. Methods : Based on the 5th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen and the results of modified Papan- icolaou staining, we divided the semen samples into groups A (normal, n = 28), B (oligoasthenoteratospermia, n = 30), and C (as- thenoteratospermia, n = 32). At I, 24, and 48 hours after sperm activation, we detected sperm PR and PR + NP by CASA and chemi- cal colorimetry, and determined sperm acrosin activity using the modified Kennedy method. Results : Sperm PR and PR + NP were significantly decreased in all the three groups at 1 - 24 hours and even more significantly at 24 - 48 hours after sperm activation as compared with the baseline (P 〈0.05). Sperm acrosin activity showed remarkable reduction in group A (P = O. 013 ), even more significant at 1 - 24 hours than at 24 - 48 hours after sperm activation, but not in groups B and C (P = 0.519 and 0.979). Conclusion : Sperm PR, PR + NP, and acrosin activity are all decreased with the extension of time after sperm activation, each in a specific manner. Examination of sperm acrosin activity should be applied as a routine tool in the assessment of male fertility.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2015年第8期733-736,共4页
National Journal of Andrology
基金
深圳市科技计划项目(医疗卫生类)(201203088)~~
关键词
男性不育
前向活动率
总活动率
顶体酶活性
male infertility
sperm motility
total sperm motility
acrosin activity