丝心蛋白支架表面处理对细胞黏附和增殖的影响

The effect of surface treatment on cell adhesion and proliferation of silk fibroin tissue engineering material

丝心蛋白(SF)具有良好的生物相容性、降解性、低或无炎症反应和来源方便等特点,被广泛用作组织工程支架材料,但其力学性能差、性质不稳定,需要对其支架进行表面处理以稳定其性能,促进细胞与支架的黏附。目前,有关SF支架处理多集中在支架表面消毒、细胞与支架之间的相互作用、支架孔径的确立、支架的表面修饰等方面。SF支架消毒主要有甲醇或乙醇浸泡,紫外线、伽马射线照射等,而甲醇的毒性和挥发性会影响细胞的存活,伽马射线设备来源和紫外线的穿透能力有限,因此乙醇浸泡成为最常用的SF支架消毒方法。细胞间相互作用,SF支架孔径和微环境,SF支架表面修饰等均可增强细胞的黏附和增殖。

Silk fibroin（SF） is a popular tissue engineering material because of its good biocompatibility, biodegradability, and low or no local inflammatory response. However, surface treatment is still needed to promote the adhesion of cells and scaffolds because SF has poor mechanical properties and is unstable. At present, surface treatments focus on surface sterilization, the interaction between cells and scaffolds, scaffold diameter, and surface modification of the SF scaffold. Sterilization methods mainly include methanol or ethanol immersion disinfection and ultraviolet and gamma ray irradiation. The toxicity and volatility of methanol affect cell survival, and gamma ray source and ultraviolet penetration abilities are limited. Hence, ethanol immersion is the most commonly used sterilization method. Other factors that can enhance the adhesion and proliferation of cells are interaction between cells, diameter and microenvironment, and surface modification of SF.