中国药典2010年版红花质量标准中山柰素含量测定方法的探讨

Investigation on analytical method of kaempferol in Carthamus tinctorius L.in Chinese Pharmacopoeia (2010 version)

目的:考察中国药典2010年版红花中山柰素含量测定色谱条件下山柰素含量测定的准确性。方法:利用二极管阵列检测器、液质联用色谱仪及核磁共振仪对山柰素主峰中包埋的干扰成分的组成及结构进行研究。结果:6-甲氧基山柰酚在中国药典2010年版红花质量标准中山柰素含量测定的色谱条件下,对山柰素的测定存在严重干扰,该分析方法不能够准确测定山柰素含量。结论:中国药典2010年版红花质量标准中山奈素含量测定方法的准确性值得商榷。

OBJECTIVE To estimate accuracy of kaempferol in Carthamus tinctorius L. as per Chinese Pharmacopoeia （2010 version）. METHODS Composition and structure of interference compounds were investigated by using diode array detector, HPLC-MS and NMR. RESULTS During analysis of kaempferol under chromatographic conditions as per Chinese Pharmacopoeia （2010 version）, 6- methoxy kaempferol seriously affected accuracy of kaempferol in Carthamus tinctorius SION Accuracy is questionable for analytical method of kaempferol in Carthamus tinctorius L.