脂肪干细胞在人工骨支架诱导下的成骨效应

Osteogenesis of adipose stem cells on artificial bone scaffold

背景:种子细胞的来源不足是限制组织重建及再生医学发展的重要因素。目的:探索脂肪干细胞与不同种类人工骨混合后的诱导成骨效果。方法:从接受美容手术的女性志愿者腹部皮下抽取液状脂肪,分离脂肪干细胞。取第4代人脂肪间充质干细胞分为单纯成骨诱导液组、成骨诱导液+羟基磷灰石组、成骨诱导液+可吸收骨材料组、成骨诱导液+重组异种骨组。后3组分别加入羟基磷灰石生物陶瓷、可吸收骨材料和重组异种骨,且每组依据干预物浓度的差异分为3,10,20 g/L 3个亚组,每3 d换液1次.共进行12 d的诱导。结果与结论:成骨诱导液+羟基磷灰石组的洗脱液钙浓度显著高于单纯成骨诱导液组(P<0.05);低浓度条件下成骨诱导液+可吸收骨材料组洗脱液钙浓度明显高于单纯成骨诱导液组(P<0.05),但高浓度成骨诱导液+可吸收骨材料组洗脱液钙浓度与单纯成骨诱导液组差异无显著性意义(P>0.05);成骨诱导液+重组异种骨组洗脱液钙浓度显著低于单纯成骨诱导液组(P<0.05)。表明不同类型人工骨支架可以影响脂肪干细胞诱导成骨效果,其中以羟基磷灰石为支架可以获得较好的诱导成骨效果。

BACKGROUND：Insufficient source of seed cel s is the important factor to restrict the tissue reconstruction and the development of regenerative medicine. OBJECTIVE：To explore the osteogenesis of adipose stem cel s cultured with different kinds of artificial bones. METHODS：Adipose tissue was extracted from female volunteers undergoing cosmetic surgery to isolate adipose stem cel s. Passage 4 adipose-derived mesenchymal stem cel s were selected and divided into osteogenic induction group, osteogenic induction＋hydroxyapatite group, osteogenic induction＋absorbable bone group and osteogenic induction＋recombinant bone xenograft group. The latter three groups were subdivided into 3, 10, 20 g/L subgroups, respectively. Culture medium was exchanged every 3 days, total y for 12 days. RESULTS AND CONCLUSION：Compared with the osteogenic induction group, the calcium concentration in the elution liquid was significantly higher in the osteogenic induction＋hydroxyapatite group and low-concentration osteogenic induction＋absorbable bone group （both P〈0.05）, but no difference was found between the osteogenic induction group and high-concentration osteogenic induction＋absorbable bone group （P〈0.05）. In addition, the calcium concentration in the elution liquid was significantly lower in the osteogenic induction＋recombinant bone xenograft group than the osteogenic induction （P〈0.05）. Therefore, different artificial bone scaffolds can influence the osteogenic effect of adipose stem cel s, and among them, hydroxyapatite has a better effect on the osteogenic induction of adipose stem cel s.