超声集成乙醇/正丁醇双水相萃取螺旋藻中的β-胡萝卜素

Ethanol / n- butanol aqueous two- phase coupled with ultrasound for the extraction of β- carotene from Spirulina platensis

采用响应面优化超声集成双水相体系萃取螺旋藻中的β-胡萝卜素最佳工艺条件。选取乙醇质量分数、正丁醇质量分数、料液比和超声时间四个因素,利用Box-Behnken Design研究各因素交互作用。结果表明优化萃取工艺条件为:乙醇质量分数8.2%,正丁醇质量分数36%,料液比为1∶20(g/m L),超声时间3 min,β-胡萝卜素的得率可达3.13 mg/g。本实验采用乙醇/正丁醇双水相萃取螺旋藻中的β-胡萝卜素将有望开发成为一项廉价高效的螺旋藻β-胡萝卜素分离提取技术。

In this study,the potential use of an aqueous two phase system（ ATPS） coupled with ultrasound for the extraction of β- carotene from Spirulina platensis was evaluated and optimized using response surface methodology（ RSM）. Box- Behnken Design was applied to evaluate the effects of four independent variables（ ethanol concentration（ %,w/w）,n- butanol concentration（ %,w/w）,ratio of material to solution（ g /m L）,ultrasonic time（ min） on β- carotene extraction yield.The optimal conditions were 8.2%,36%,1∶20（ g / m L） and 3 min for ethanol concentration,n- butanol concentration,ratio of material to solution and ultrasonic time respectively.β- carotene yield was determined to be 3.13 mg / g under the optimal conditions.The results indicated that Spirulina platensis β- carotene would be purified by a simple,efficient extraction technology using inexpensive ethanol /n- butanol ATPS.