火棘果水溶性膳食纤维酶法制备工艺及单糖成分和物化性质研究

Study on the monosaccharide compositions,physico- properties and preparation technology of soluble dietary fiber by enzymatic method from Pyracantha Fortuneana

以野生火棘果为原料,采用酶法制备火棘果水溶性膳食纤维(SDF)。以单因素纤维素酶添加量、酶解时间和液料比为实验因素,以水溶性膳食纤维得率为响应值,采用三因素三水平的响应面分析法优化提取工艺条件。同时,利用气相色谱法研究了火棘果膳食纤维的单糖组成和物化性质。结果表明,酶法制备火棘果水溶性膳食纤维的最佳工艺条件为:酶添加量0.83%,酶解时间2 h,液料比23 m L/g,在此条件下火棘果SDF的得率预测值为5.97%,实际验证值为5.91%。火棘果膳食纤维的单糖成分主要为木糖、阿拉伯糖、鼠李糖、山梨糖,同时还含有少量的果糖和甘露糖,其中以木糖含量最高,达33.56%。物化性质实验表明,火棘果膳食纤维具有良好的持水性和持油性,同时对葡萄糖也具有较强的吸收能力。

The enzymatic method was adopted to extract soluble dietary fiber from Pyracantha Fortuneana. The experimental factors including the amount of cellulose,extract time and liquid- to- solid ratio on the extraction ratio of soluble dietary fiber（ SDF） was adopted.Meanwhile,the monosaccharide compositions and physico- properties of the dietary fiber from Pyracantha Fortuneana was researched. The results showed that,the optimal extraction conditions of the soluble dietary fiber prepared by enzymatic method were as follows： the amount of cellulose was0.83%; extract time was 2 h and the liquid- to- solid ratio was 23 m L / g,the predicted value and measured value of the soluble dietary fiber were 5.97% and 5.91%,respectively.The main monosaccharide compositions of the dietary fiber from Pyracantha Fortuneana were xylose,arabinose,rhamnose and sorbose,also contained a small amount of fructose and mannose,among them with xylose content was the highest,reached to 33.56%. The results of the physico- properties experiments showed that,the dietary fiber from Pyracantha Fortuneana possessed good WHC and OHC,and had strong glucose- adsorption capacity.