麦胚无细胞蛋白合成系统研究进展

Research progress in wheat germ cell- free protein synthesis system

小麦胚芽作为面粉加工过程中的副产物,具有很高的生物活性,其中包含许多蛋白质合成必需的活性成分,如蛋白质合成场所、蛋白因子及其他相关酶系等。本文综述了在后基因组时代,随着生物技术的发展,以小麦胚芽为原料的无细胞蛋白合成系统在结构蛋白、毒性蛋白和功能蛋白表达方面的优势,阐述了其在蛋白质组学研究和生物制药等领域的重要应用潜力,以期小麦胚芽的深层次开发利用提供了重要依据。

In this paper,Wheat germ,a by- product of flour milling,possesses high bio- active and multiple functional compositions,such as the translational apparatus,translation factors and enzymes,which are essential for protein synthesis.With the development of biotechnology in the post genome era,the advantages of wheat germ cell- free protein synthesis system were showed in the expression of structural,toxic and functional proteins. This system was widely applied in proteomics research and the biopharmaceutical field,which provide strong evidence for the importance of developing derived products of wheat germ.