建立麻疯树提取物中佛波酯的高效液相色谱测定方法

Determination of phorbol ester in Jatropha curcas L extracts using HPLC

目的：建立高效液相色谱测定麻疯树提取物中佛波酯含量的方法。方法：直接用二氯甲烷溶液稀释后,过滤、进样,流动相：乙腈/水（梯度洗脱）,柱温25℃,流速1.0 ml/min,检测波长280 nm,标准曲线范围15.6~250.0μg/m L,相关系数R=0.9999,检测时间40 min以内提取麻疯树提取液样品。结果：佛波酯标准曲线范围15.6~250.0μg/m L,相关系数R=0.9999,加样平均回收率91.2%-94.6%,精密度RSD 1.3%,检出限5.0μg/m L。结论：该分析方法准确、样品处理方法简单、快速,适合大批量麻疯树提取物中佛波酯含量测定。

Objective： To develop a method for determination of phorbol ester in Jatropha curcas L extracts by HPLC. Meth-ods： Phorbol ester extract samples were diluted with CHCl3, filtered and injected into HPLC. The chromatographic separation was a-chieved on a reverse phase C18 column with gradient elution, using a mobile phase of acetonitrile/ water; detector wave was set at 280nm, column temptation was 25℃ , flow rate was 1. 0 ml/ min, and linear range was 15. 6-250 μg/ ml. Detection time was less than 40min. Results： The cablication curves of phorbol ester showed good linearity with correlation coefficient of 0. 9999. The mean recov-ery of spiked samples were from 91. 2% ~ 94. 6% . The RSD of precision was 1. 3% . The detection limits of phorbol ester was 5. 0μg/ mL. Conclusions： The results show that the method is simple and sensitive, and can be applied to routine analysis of phorbol ester in Jatropha curcas L extracts.