大鼠帕金森病模型中鱼藤酮对黑质多巴胺能神经元影响

The Effect of Rotenone on Dopaminic Neurons of Brain in Rat Model of Parkinson's Disease

目的研究鱼藤酮对大鼠脑黑质多巴胺能神经体系及氧化应激参数(丙二醛和谷胱甘肽)的影响,探讨鱼藤酮对多巴胺能神经元的毒性作用及其发病机制。方法实验组10只大鼠背部皮下注射鱼藤酮1.0 mg/(kg·d),对照组10只大鼠背部皮下注射葵花籽油1 m L/kg,两组均注射50 d。以免疫细胞化学技术检测大鼠脑内酪氨酸羟化酶(TH)的表达改变,并采用分光光度法检测大鼠脑内氧化应激参数(丙二醛和谷胱甘肽)的改变。结果大鼠多巴胺能神经元有不同程度的损伤,大鼠脑内TH免疫反应强度在黑质和纹状体都有明显的降低,实验组黑质TH免疫反应阳性神经元数目明显少于对照组(P<0.01);分光光度计法检测中脑黑质中谷胱甘肽,在实验组中较对照组明显减低,差异有统计学意义(P<0.01),丙二醛在实验组中较对照组明显增高,差异有统计学意义(P<0.01)。结论鱼藤酮具有明显的神经毒性,能导致大鼠脑内多巴胺能神经元的损伤,导致大鼠脑内发生明显氧化应激损伤。

Objective To study the effect of rotenone on rat substantia nigra dopamine （DA） in the nervous system and oxidative stress parameters （malondialdehyde and glutathione）, the influence of rotenone on DA neurons toxic effect and its pathogenesis. Methods This study applied back subcutaneous injection of rotenone in rats [1.0 mg/（kg · d）], and used immunocytochemistry technique to detect changes in the expression of tyrosine kinase （TH） in 10 rats of the control group and 10 rats of the experimental group. Spectrophotometry was used to detect the change of oxidative stress parameters in rats （malondialdehyde and glutathione）. Results The TH immune response strength of rats in the substantia nigra DA neurons in rats had various degrees of damage. and striatum decreased significantly. The number of immune response nigra TH positive neurons was significantly less in the experimental group than in the control group （P 〈 0.01）. Spectrophotometer method was used to detect the midbrain nigra of glutathione, which was significantly less in the experimental group than in the control group （P - 0.01）. Malondialdehyde in the experimental group was significantly higher （P 〈 0.01）. Conclusion Rotenone has obvious neurotoxicity, and can lead to the damage of DA neurons and obvious oxidative stress injury in rats, which provides an experimental basis for the pathogenesis of Parkinson＇s disease, and at the same time provides new targets for the treatment.