血清5-羟色胺及其受体基因T102C多态性与非抑郁症患者服毒自杀行为的相关研究

Correlation of the T102C Polymorphism of Serum 5-Hydroxytryptamine and Its Receptor Gene and Suicidal Behavior by Poison in Non-depressive Patients

目的探讨非抑郁症服毒自杀患者血清5-羟色胺(5-HT)水平及其2A受体基因T102C多态性与服毒自杀的关系,以早期识别及干预服毒自杀行为。方法 2013年1月-2014年6月选取62例既往未诊断抑郁症、有服毒自杀行为的患者为病例组,另同期选取66例健康者作为对照组。采集外周血标本,利用酶联免疫吸附试验测定血清5-HT水平;采用聚合酶链限制性片段长度多态性法测定5-HT受体基因2A-T102C多态性。数据资料均采用SPSS 19.0统计分析软件进行统计分析。结果病例组全血5-HT水平明显低于对照组,差异有统计学意义(P<0.001);病例组C等位基因频率(0.55)高于健康对照组(0.40),差异有统计学意义(χ2=5.533,P=0.013);基因型分布也有差异,突变纯合子CC基因型病例组(0.29)较对照组(0.12)高,差异有统计学意义(χ2=5.648,P=0.017)。结论血清5-HT水平及2A受体基因T102C多态性、C等位基因频率与非抑郁症患者服毒自杀行为可能存在关联。

Objective To explore the correlation of serumLevels of 5-hydroxytryptamine （5-HT） and 5-HT2A receptor gene T102C polymorphism with suicidal behavior by poison in non-depressive patients, in order to diagnose and intervene the suicidal behavior of patients as early as possible. Methods Sixty-two non-depressive patients with the behavior of suicide by poison treated between January 2013 and June 2014 were selected as patient group, and other 66 healthy persons were selected as control group. Peripheral blood in the two groups of patients were collected to test the serumLevels of 5-HT with enzyme linked immunosorbent assay, and the 5-HT2A receptor gene T102C polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism. The data were analyzed by SPSS 19.0 statistically. Results The serumLevel of 5-HT in the patient group was significantly lower than that in the control group （P 〈 0.001）, and the frequency ofT102C genotype in the patient group was higher than the control group （χ^2=5.533, P=0.013）. The distribution of genotype was different, and homozygous mutations of CC in the patient group were higher than the control group （χ^2=5.648, P=0.017）. Conclusion The serumLevels of 5-HT and 5-HT2A receptor gene T102C polymorphism, and the frequency of T102C genotype may be related to suicidal behavior by poison in non-depressive patients.