芹菜素对人肺癌细胞株A549迁移和侵袭能力的抑制作用及机制

Inhibitory effect of apigenin against migration and invasion of human lung cancer A549 cells and the related mechanism

目的探讨芹菜素对肺癌A549细胞迁移和侵袭能力的影响和机制。方法采用0、5、10、20、40、80、160μmol/L浓度的芹菜素培养液与A549细胞共培养,利用CCK8法检测A549细胞的生存率,Transwell迁移和侵袭实验检测芹菜素对A549细胞迁移和侵袭能力的影响,酶联免疫吸附实验（ELISA）、蛋白质印迹法分析芹菜素对细胞基质金属蛋白酶（MMP-2、MMP-9）和血管内皮生长因子（VEGF）活性和蛋白表达的改变。结果 CCK8显示40-160μmol/L的芹菜素可抑制A549细胞增殖,并呈浓度依赖性（P〈0.05）;与对照组比较,经芹菜素处理后A549细胞迁移和侵袭能力降低,相对应的MMP-2、VEGF蛋白表达和分泌减少（P〈0.05）;但对于MMP-9,芹菜素不能抑制其蛋白的表达和分泌。结论芹菜素可有效抑制肺癌A549细胞迁移和侵袭能力,其机制可能与芹菜素降低细胞MMP-2、VEGF的蛋白表达和分泌相关。

Objective To evaluate the effect of apigenin on cell migration,invasion of human lung cancer cell line A549 and the underlying mechanisms.Methods A549 cells were cultured with different concentrations of apigenin（0,5,10,20,40,80,and 160μmol/L）,and CCK8 assay was used to evaluate the cell inhibition rates.Cell migration and invasion capabilities were evaluated by means of transwell and transwell matrix penetration assays.Enzyme-linked immunosorbent assay（ELISA）and Western blotting analysis were used to study the activities and expressions of invasion-related proteins matrixmetallopeptidase 2（MMP-2）,MMP-9and vascular endothelial growth factor（VEGF）.Results CCK8 assay results showed that apigenin caused a concentration-dependent（40,80,and 160μmol/L）inhibition against proliferation of A549cells（P0.05）.Compared with the control group,apigenin significantly inhibited the migration and invasion capabilities of A549 cells,accompanied by significantly decreased MMP-2and VEGF expression（P0.05）.However,apigenin showed no effect on MMP-9protein expression.Conclusion Apigenin can effectively inhibit migration and invasion of human lung cancer cell line A549,which is probably through suppressing the expressions of MMP-2and VEGF.