趋化因子受体CXCR4核定位表达与肾癌转移及预后的相关性

Nuclear expression of CXCR4 in renal cancer correlates with metastasis and poor survival

目的探讨肾癌组织趋化因子受体CXCR4核定位表达与肾癌转移及预后的相关性。方法采用免疫荧光染色法检测413例肾癌组织CXCR4的亚细胞定位表达,根据染色结果分为核定位阳性表达组及核定位阴性表达组,对比分析两组患者的临床资料及预后。结果 413例肾癌组织中170例CXCR4核定位阳性表达,243例CXCR4核定位阴性表达,两组基线资料差异无统计学意义。统计分析结果表明:与核定位阴性表达组相比,核定位阳性表达组Robson分期高、癌栓发生率高、淋巴结转移率高、远处转移率高,差异均有统计学意义(P<0.01)。核定位阳性表达组生存率为86.5%(147/170),低于核定位阴性表达组97.1%(236/243),差异有统计学意义(P<0.001)。结论肾癌组织中CXCR4核定位表达与Robson分期高、癌栓形成、淋巴结转移、远处转移及患者预后差相关。

Objective To investigate the nuclear expression of CXC chemokine receptor 4（CXCR4）in renal cancer,and analyze its relation with renal cancer metastasis and prognosis.Methods A total of 413 patients with renal cancer who were treated in our urologic center from Mar.2011 to Nov.2012 were included in the present study.The subcellular expression of CXCR4 was examined by immunofluorescence staining;the correlation between CXCR4 nuclear location and clinical features,prognosis was analyzed.Results We found that 170 of the 413 renal cancer patients were CXCR4 nuclear staining-positive（group A）,and the rest 243 cases were CXCR4 nuclear staining-negative（group B）;the two groups had matchable baseline data.Compared with group B,group A had significantly higher Robson stage（P0.01）,more frequent cancer embolus（P0.01）,more frequent lymphatic metastasis（P0.01）,and more frequent distant metastasis（P0.01）.The overall survival rate of group A（86.5%,147/170）was significantly lower than that of group B（97.1%,236/243;P0.001）.Conclusion Nuclear expression of CXCR4 in renal cancer tissues is associated with higher Robson stage,more frequent cancer embolus,more frequent lymphatic metastasis,more frequent distant metastasis and poor prognosis.