坤泰胶囊对大鼠实验性子宫异位内膜生长及其妊娠结局的影响

Effect of Kuntai capsule on ectopic endometrium and the pregnancy outcome in a rat of endometriosis

目的:研究坤泰胶囊(KTC)对SD大鼠实验性子宫异位内膜生长及其妊娠结局的影响。方法:手术法建立SD大鼠子宫内膜异位症(EMs)模型。将造模成功的SD大鼠分为模型对照组、阳性对照组(每只给予醋酸亮丙瑞林0.1 mg皮下注射,共1次),KTC低剂量组(每只按0.24 g/kg体质量灌胃,qd×21 d)、KTC高剂量组(每只按0.60 g/kg体质量灌胃,qd×21 d),每组14只。给药21 d后,每组解剖8只,比较给药前、后异位内膜体积变化;Western blotting法测定异位内膜caspase-8、caspase-3和caspase-9蛋白表达。每组剩余6只与雄鼠交配,妊娠12 d后,解剖观察受孕情况,并对胎盘、胎仔称量。动情周期正常的SD大鼠14只作为空白对照。结果:与模型对照组相比,KTC低、高剂量组体质量无统计学差异(P>0.05);KTC低、高剂量组异位内膜抑制率分别为(40.25±35.18)%和(65.52±15.82)%;KTC低、高剂量组异位内膜中caspase-8、caspase-9和活化caspase-3明显升高(P<0.05);KTC低剂量组的大鼠胎仔数量呈增加趋势。结论:KTC通过升高caspase-8、caspase-9和活化caspase-3蛋白含量,促进异位内膜的凋亡;KTC低剂量组EMs大鼠受孕率呈现出升高的趋势。

Objective： To investigate the effect of Kuntai capsule（KTC） on endometriosis（EMs） the growth of ectopic endometrium and the pregnancy outcome in SD rats. Methods： Rat model of EMs was established with surgically implanted endometrium, and then the animals were given different doses of KTC in 4 groups of 14 rats each. After 3 weeks of treatment, laparotomy was performed to examine the volume changes in the ectopic endometrium for 8 rats per group. Western blotting assay was used to test the protein level of caspase-8, caspase-9 and caspase-3 in ectopic endometrium. The remaining 6 rats per group were mated with male rats. After successful pregnancy for 12 d, the fetal rats and placenta were weighed. Results： Compared with the model group, the inhibitory rates were（40.25±35.18）% and（65.52±15.82）%, respectively in the group of KTC 0.24 g/kg and 0.60 g/kg, qd×21 d; KTC induced apoptosis in ectopic endometrium, through increasing the protein expression of caspases-8 and caspases-9, activated caspase-3. The pregnancy rate increased by 67% and the average number of fetus increased by 2 times in KTC low-dose group compared with the model group.Conclusion： The KTC inhibited the growth of ectopic endometrial tissue via improving the expression of caspase-8,caspases-9, activated caspase-3, and the pregnancy rate was improved in the group of KTC 0.24 g/kg treatment.