贵州省县级和省级疟疾诊断实验室检测结果比较分析

Comparison of Malaria Diagnosis between County and Provincial Laboratories in Guizhou Province

贵州省省级疟疾诊断实验室通过PCR法和镜检法对各县级疟疾诊断实验室上报的疟疾网报病例进行复核,以PCR和镜检一致的结果作为判定依据,对县级疟疾诊断实验室镜检结果进行评价。共对89份血样进行复核,PCR检出24份阳性,15份为恶性疟原虫,7份为间日疟原虫,2份为卵形疟原虫,均为输入性病例;省级镜检和PCR结果一致的有21份。县级与省级检测结果的总符合率为79.8%(67/84),县级漏检率为9.5%(2/21),误检率为23.8%(15/63),两级实验室检测的Kappa值为0.6,属于中、高度一致。

Malaria cases reported by county laboratories were further tested in the provincial laboratory in Guizhou province by using PCR test and microscopy. The consistency between PCR and microscopic results in the provincial laboratory was set as the basis for evaluation of microscopic results in county laboratories. In 89 samples, 24 were identified by PCR to be positive for malaria, among which 15 were infected with P. falciparum, 7 with P. vivax, and 2 with P. ova/e; all were imported cases. And 21 samples had consistent identifications by PCR test and microscopic examination in the provincial laboratory. The total coincidence rate between county and provincial laboratories was 79.8%（67/84）, and the undetected and error rates in county laboratories were 9.5%（2/21） and 23.8% （15/63）, respectively. The Kappa value between county and provicial diagnosis was 0.6, being at the medium-to-high level of consistentcy.