MicroRNA靶序列IL-13基因多态性与大肠癌发生及预后关系

Association of microRNA target sequence polymorphisms in IL-13 gene with the risk and prognosis of colorectal cancer

目的 研究microRNA靶序列白素介-13（interleukin-13,IL-13）基因多态位点对大肠癌发生和预后的影响。方法 采用病例对照研究方法分析其与大肠癌发病的关系,并探讨多态位点与饮食因素的联合和交互作用与大肠癌发生的关系;随访309例大肠癌患者,探讨其多态性与大肠癌预后的关系。运用生物信息学软件预测mi-croRNA靶序列IL-13基因多态位点,为rs847、rs848和rs1295685。TaqMan探针方法对513例病例和576例对照进行基因分型。结果 IL-13基因多态性与饮食因素的联合和交互作用与大肠癌的发病有关。3个多态位点携带突变基因与隔夜食品的摄入有协同交互作用,其OR值分别为rs847（G/A）（OR=1.25,95%CI：1.08-1.43）,rs848（G/T）（OR=1.24,95%CI：1.08-1.42）,rs1295685（C/T）（OR=1.21,95%CI：1.05-1.39）。预后研究表明,IL-13基因的3个位点与大肠癌预后无统计学关联,但是大肠癌的大体分型,Dukes’分期和是否转移都与大肠癌预后生存有关。结论 IL-13基因多态性与饮食因素（隔夜食品和刺激性食品）的联合和交互作用与大肠癌的易感性有关。

Objective To explore the association of microRNA target sequence polymorphisms in IL-13 gene with the risk and prognosis of colorectal cancer（ CRC）. Methods A case-control study and a follow-up study including 309 cases were conducted to explore the associations of three polymorphisms in IL-13 with the risk and prognosis of CRC,as well as the combinations and interactions of polymorphisms with dietary factors on CRC risk. Bioinformatics tools were used to predict polymorphic loci of microRNA target sequence polymorphisms rs847,rs848 and rs1295685 in IL-13 gene. TaqMan SNP Genotyping Assay was used to analyze these SNPs in genomic DNA samples for 513 CRC patients and 576 cancerfree controls. Results The combination and interaction of IL-13 gene polymorphism and dietary factors was associated with the onset of colorectal cancer. Significant synergistic interactions were observed for rs847（ G / A）（ OR = 1. 25; 95% CI：1. 08-1. 43）,rs848（ G / T）（ OR = 1. 24; 95% CI： 1. 08-1. 42）,rs1295685（ C / T）（ OR = 1. 21; 95% CI： 1. 05-1. 39） between the three SNPs and overnight food intaking on the risk of colorectal cancer,respectively. Prognosis study showed that there were no significant differences between the three SNPs and colorectal cancer prognosis,but the general classification of colorectal cancer,stage of Dukes＇,and metastasis had impacts on the overall survival of CRC. Conclusions The combinations and interactions of IL-13 gene polymorphisms and dietary factors（ overnight and pungent food） are associated with the susceptibility of colorectal cancer.