用于巨型脂质体制备及收集的微流控芯片研究

Microfluidic Chips for Preparation and Collection of Giant Vesicles

构建了一种集成制备和收集功能的微流控芯片系统。利用微流控技术将脂质溶液输入微电极阵列中形成脂质膜,在微电极阵列产生的聚焦电场作用下,高效形成一定粒径范围的巨型脂质体,稳定的巨型球形脂质体的最高产率可以达到60%;然后将制备的脂质体经微通道输送到收集腔室,通过重力作用和微孔滤膜的筛选可得到90%以上,尺寸在10"50#m的稳定巨型球形脂质体。此芯片系统有效地克服了目前巨型脂质体制备方法效率低、脂质体粒径分布广、难筛选和难收集等缺点。

A microfluidic chip with the preparation module and collection module was fabricated, in which mierofluidic technology and microelectrode array were used for the preparation of giant vesicles. First, lipid solution was loaded into the microeleetrode array through microchannels to form lipid film, then an electric field was subsequently loaded on the microelectrode array and the giant vesicles with a controlled diameter were formed efficiently. The ratio of the formed stable giant spherical vesicles could reach up to 60%. Giant vesicles and other materials were flushed into the upper layer of the collection chamber by microehannel. 90% of the stable giant spherical vesicles with 10-50 txm diameter could be sorted in the upper layer of the collection chamber by using micropore filter and gravity depositing. This microfluidic chip could overcome some defects existing in the current preparation method such as low efficiency, wide distribution of diameters, as well as difficult for screening and collection.