三氯化铟对人正常肺上皮细胞增殖的影响及其机制研究

Effects of indium chloride on proliferation of human lung epithelial cells and its mechanism

目的探讨不同浓度三氯化铟（InCl3）对人正常肺上皮（Beas-2B）细胞增殖的影响及其可能机制。方法不同浓度（0、0．3、1．0、3．0、10．0、30．0、90．0、270．0、810．0μmol／L）InCl3分别处理Beas-2B细胞24、48和72h，用CCK-8法检测InCl3对细胞增殖的影响；用Annexin V-PI双染，经流式细胞术检测InCl3对细胞凋亡的影响；以2’，7’-二氯荧光黄双乙酸盐为探针，用流式细胞术检测InCl3染毒后Beas-2B细胞内的活性氧（ROS）水平。结果0．3～3．0μmol／LInCl3组的细胞存活率明显升高，而30．0～810．0μmol／LInCl3组72h后细胞存活率降低，与对照组相比，差异有统计学意义（P〈0．05）；0．3μmol／LInCl3组在72h内都未能引起细胞凋亡的发生，而30．0和810．0μmol／LInCl3组72h后引起明显的细胞早期凋亡，0.3μmol/LInCl3组72h内ROS含量与对照组相比略微下降，而30．0和810．0μmol/LInCl3组72h后，细胞内ROS含量明显增加，差异有统计学意义（P〈0．05）。结论低浓度InCl3可能通过降低细胞内ROS促进细胞增殖，而高浓度InCl3则通过引起细胞内ROS升高、细胞凋亡而发挥其抑制细胞活力的作用。

Objective To investigate the effects of different concentrations of indium chloride （InCl3） on the proliferation of human lung epithelial （Beas-2B） cells and its potential mechanism. Methods Beas-2B cells were exposed to different concentrations of InCl3 （0.3, 1.0, 3.0, 10.0, 30.0, 90.0, 270.0, and 810.0 μmol/L） for 24, 48, and 72 h, respectively. Tbe effects of InCl3 on cell proliferation were determined by the CCK-8 assay. The effects of InCl3 on apoptosis were evaluated using annexin V-PI staining followed by flow cytometry. The level of intracellular reactive oxygen species （ROS） in Beas-2B ceils after exposure to InCl3 was determined using 2＇, 7＇-dichlorofluorescein diacetate labeling followed by flow cytometry. Results Compared with the control group, InCl3 at a relatively low concentration （0.3～3.0 μmol/L） significantly promoted cell proliferation （P〈0.05）, while InCl3 at a relatively high concentration （30.0～80.0μmol/L） significantly inhibited cell proliferation after 72 h （P〈0.05）. InCl3 at a concentration of 0.3 μmol/L failed to induce apoptosis within 72 h; however, InCl3 at a concentration of 30.0 or 810.0 μmol/L induced substantial early apoptosis after 72 h. Compared with the control group, cells exposed to 0.3 μmol/L InCl3 showed a slight decrease in the level of intracellular ROS within 72 h, while cells exposed to 30.0 or 810.0 μmol/L InCl3 showed a significant increase in the level of intracellular ROS after 72 h （P〈0.05）. Conclusion At a low concentration, InCl3 stimulates cell proliferation by reducing intracellular ROS, However, at a high concentration, InCl3 inhibits cell viability by elevating intracellular ROS and inducing apoptosis.