姜黄素拮抗百草枯染毒大鼠脂质过氧化作用的研究

Antagonistic effect of curcumin on lipid peroxidation of rats poisoned by paraquat

目的观察姜黄素治疗条件下百草枯（PQ）中毒大鼠脂质过氧化的变化。方法将清洁级8周龄Wistar雄性大鼠50只随机分为高剂量姜黄素＋常规治疗组、低剂量姜黄素＋常规治疗组、单独高剂量姜黄素治疗组、PQ单纯染毒组和空白对照组，分别在染毒后第1、3、7、14天和第21天检测大鼠血清中谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活力和丙二醛（MDA）含量。结果与空白对照组相比，高剂量姜黄素＋常规治疗组、低剂量姜黄素＋常规治疗组、单独高剂量姜黄素治疗组、PQ单独染毒组大鼠MDA含量明显升高，而SOD、GSH-Px和CAT活力明显降低，差异均有统计学意义（P〈0．05）。高剂量姜黄素＋常规治疗组、低剂量姜黄素＋常规治疗组和单独高剂量姜黄素治疗组与PQ单独染毒组比较，MDA含量降低，SOD、GSH．Px、CAT活力升高，差异有统计学意义（P〈0．05）。高剂量姜黄素＋常规治疗组脂质过氧化指标改善最明显。结论姜黄素可明显降低大鼠血清脂质过氧化水平，抑制并延缓机体损伤的发生发展。

Objective To explore the pathogenesis of paraquat poisoning and observe the change in lipid peroxidation of rats treated with different doses of curcumin. Methods A total of 50 8-week-old male Wistar rats （clean grade ） were randomly divided into high-dose curcumin plus conventional treatment group, low-dose curcumin plus conventional treatment group, high-dose curcumin treatment group, poisoned group, and blank control group. Glutathione peroxidase （GSH-Px）, catalase （CAT）, superoxide dismutase （SOD）, and malondialdehyde （MDA） levels in rat serum were measured at 1, 3, 7, 14, and 21 d post paraquat injection. Results Compared with the blank control group, other groups had significantly higher MDA levels but lower SOD, GSH-PX, and CAT activities. The high-dose, low-dose curcumin plus conventional treatment, and high- dose curcumin treatment groups had significantly lower serum lipid peroxidation levels compared with the poisoned group and among them the high-dose curcumin plus conventional treatment group had the most significant improvement. Conclusion Curcumin can significantly decrease serum lipid peroxidation level in rats and inhibit and delay the occurrence and progression of the damage to the body.