环氧化酶-2与p53在恶性黑色素瘤组织中的表达意义及相关性分析

Clinical value of expressions of COX-2 and P53 in malignant melanoma

目的探讨恶性黑色素瘤组织中环氧化酶-2(COX-2)与p53的表达,明确其在恶性黑色素瘤发生、发展中的临床意义。方法收集恶性黑色素瘤组织和色素痣患者各30例,采用免疫组织化学检测组织中COX-2和p53表达,并与临床资料进行相关性分析。结果 COX-2和p53蛋白在恶性黑色素瘤组织中表达阳性率高于色素痣组(P<0.01)。恶性黑色素瘤组织中COX-2与p53的表达呈正相关(r=0.452,P=0.015)。恶性黑色素瘤中COX-2表达在年龄、性别分组中,差异无统计学意义(P>0.05)。而恶性黑色素瘤病理分期Ⅲ、Ⅳ期患者的COX-2阳性率显著高于Ⅰ、Ⅱ期患者(P<0.01);有淋巴结转移患者的COX-2阳性率高于无淋巴结转移患者(P<0.05)。结论 COX-2可能为判定MM恶性程度的一种标志,可能通过p53突变,导致COX-2表达增强,从而在恶性黑色素瘤中发生作用。

[Objective] To observe the clinical value of the expressions of COX-2 and P53 in malignant melanoma （MM）. [Methods] Thirty patients with MM and thirty cases of nevus were collected. The expressions of COX-2 and P53 were detected by immunohistochemical method and the correlation between the expression of COX-2 and clinical data was analyzed. [Results] The expressions of COX-2 protein and P53 protein in MM tissue were statistically higher than those in nevus （P〈 0.01）. There was a statistically significant positive correlation between the expression of COX-2 and P53 （r = 0.452, P 〈 0.05）. There was no statistically diffe- rences of COX-2 expression in MM patients with different age and gender （P 〉 0.05）, while the positive rate of COX-2 in the stage III and IV MM patients was significantly higher than that in the stage I and II patients （P〈 0.01）. The positive rate of COX-2 in the patients with lymph node metastasis was higher than that in the patients without lymph node metastasis （P〈 0.05）. [Conclusion] COX-2 may become a new symbol for judging the malignant degree of malignant melanoma, which is supposed to be activated by p53 mutation.