刺激植物响应蛋白基因TatEpl1的克隆及原核表达载体构建

Cloning and Prokaryotic Expression Vector Construction of Eliciting Plant Response Protein Gene TatEpl1

以深绿木霉(Trichoderma atroviride)ACCC30153为试材,采用PCR技术克隆到刺激植物响应蛋白基因TatEpl1,并构建TatEpl1的原核表达载体。结果表明:经测序得到的cDNA和DNA序列长度分别为417bp和487bp,接受号分别为JN695780和JN695781。以cDNA为模板进行PCR获得TatEpl1基因片段,并将目的片段插入原核表达载体pGEX-4T-2的相应位置,获得重组表达载体pGEX-TatEpl1,并将其转入大肠杆菌(Escherichia coli)BL21中获得重组菌株BL21-TatEpl1,经检测均呈阳性。

Using the T.atroviride ACCC30153 as material,the eliciting plant response protein gene TatEpl1 was cloned by PCR,and the prokaryotic expression vector of TatEpl1 was constructed.The results showed that,the cDNA sequence of TatEpl1 was 417bp in length,and the DNA sequence was 487 bp in length and contained two exons and one intron.The cDNA and DNA sequences of TatEpl1 were deposited in the GenBank database with the accession numbers JN695781 and JN695780,respectively.The segment of TatEpl1 was inserted into prokaryotic expression vector pGEX-4T-2and transformed into E.coli BL21 for obtaining the recombinant strain BL21-TatEpl1.The results showed all of the transformants were positive by detection.