重离子与X射线辐照对人舌鳞状细胞癌Tca8113细胞株CXCR4表达的影响

Effects of Heavy Ion Beams and X-ray on CXCR4 Expression of Human Tongue Squamous Cell Carcinoma Tca8113 Cell Line

目的:以CXCR4mRNA的表达量为指标探讨不同辐射方式对Tca8113细胞株细胞侵袭能力的影响。方法:采用不同剂量的重离子束与X-ray对细胞进行辐射,采用荧光实时定量PCR法观察细胞中CXCR4mRNA表达量的变化。结果:与空白对照组比较,在相同辐射剂量下,X线辐照组细胞中CXCR4的表达量在8h时较低,于12h达到高峰,该峰值在1Gy、4Gy组较为显著(P<0.05),在2Gy组则差异不明显;而重离子辐照组细胞中CXCR4的mRNA表达水平在辐照后12h内均明显降低(P<0.05)。两者在24h的表达基本恢复至空白对照组水平。在相同时间点时,重离子组CXCR4的表达量与X线组比较则有显著降低(P<0.01)。结论:与常规X线相比,重离子对体外培养的人舌鳞癌细胞中CXCR4的表达的抑制能力更强,提示重离子抑制舌鳞癌细胞侵袭的能力优于常规X射线。

Objective： To study the effects of heavy ion beams and X-ray on the expression of chemokine receptor CXCR4 in Tca8113 cell line, and explore the underlying mechanism. Methods： The mRNA express level of CXCR4 was detected by real-time PCR after Tca8113 ceils were treated. Results： Compared with the control group, the expression of CXCR4 in the X-ray group got to the lowest level at 8 h, and reached the peak value at 12 h （P〈0. 05） after radiation. In addition, the changes in the 1 Gy and 4 Gy groups were more obvious （P〈0.05）, but not significant in the 2Gy group. The expression of CXCR4 was significantly down-regulated at 12 h after heavy ion beams treatment. Meanwhile, the expression of CXCR4 in the 1 Gy and 4 Gy groups returned to the control level at 24 h after radiation. Compared with X-ray group, CXCR4 expression was obviously lower in the heavy ion beams group （P〈0.01）. Conclusion： Heavy ion beams may be a better choice to inhibit the invasion of Tca8113 cell line in vitro.