摘要
目的:以CXCR4mRNA的表达量为指标探讨不同辐射方式对Tca8113细胞株细胞侵袭能力的影响。方法:采用不同剂量的重离子束与X-ray对细胞进行辐射,采用荧光实时定量PCR法观察细胞中CXCR4mRNA表达量的变化。结果:与空白对照组比较,在相同辐射剂量下,X线辐照组细胞中CXCR4的表达量在8h时较低,于12h达到高峰,该峰值在1Gy、4Gy组较为显著(P<0.05),在2Gy组则差异不明显;而重离子辐照组细胞中CXCR4的mRNA表达水平在辐照后12h内均明显降低(P<0.05)。两者在24h的表达基本恢复至空白对照组水平。在相同时间点时,重离子组CXCR4的表达量与X线组比较则有显著降低(P<0.01)。结论:与常规X线相比,重离子对体外培养的人舌鳞癌细胞中CXCR4的表达的抑制能力更强,提示重离子抑制舌鳞癌细胞侵袭的能力优于常规X射线。
Objective: To study the effects of heavy ion beams and X-ray on the expression of chemokine receptor CXCR4 in Tca8113 cell line, and explore the underlying mechanism. Methods: The mRNA express level of CXCR4 was detected by real-time PCR after Tca8113 ceils were treated. Results: Compared with the control group, the expression of CXCR4 in the X-ray group got to the lowest level at 8 h, and reached the peak value at 12 h (P〈0. 05) after radiation. In addition, the changes in the 1 Gy and 4 Gy groups were more obvious (P〈0.05), but not significant in the 2Gy group. The expression of CXCR4 was significantly down-regulated at 12 h after heavy ion beams treatment. Meanwhile, the expression of CXCR4 in the 1 Gy and 4 Gy groups returned to the control level at 24 h after radiation. Compared with X-ray group, CXCR4 expression was obviously lower in the heavy ion beams group (P〈0.01). Conclusion: Heavy ion beams may be a better choice to inhibit the invasion of Tca8113 cell line in vitro.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2015年第8期789-792,共4页
Journal of Oral Science Research
基金
国家自然科学基金(编号:81260403)
甘肃省科技支撑计划(编号:1304FKCA092)
关键词
重离子
X射线
舌鳞癌
CXCR4
肿瘤侵袭
Heavy ion beams
X-ray
Tongue squamous cell carcinoma
CXCR4
Tumor invasion