细辛长期毒性对SD大鼠心电图及心肌酶谱的影响

Effects of Long Term Toxicity of Asarum on SD Rat's ECG and Myocardial Enzyme Spectrum

目的观察细辛长期毒性对SD大鼠心电图及心肌酶谱的影响。方法将SD大鼠随机分为空白对照组(蒸馏水)和细辛低、中、高剂量组(0.27、0.81、1.35 g/kg),每日灌胃1次,连续28d。分别于给药第0、14、28天测定各组Ⅱ导联心电图,并计算心率,给药第28天采集血液测定心肌酶谱。结果与对照组比较,细辛低剂量组心电图及心肌酶各指标无明显变化;中、高剂量组心率减慢,中剂量组可显著引起肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、羟丁酸脱氢酶(HBDH)含量升高;高剂量组LDH含量升高,CK、CK-MB和HBDH含量恢复至正常。结论长期大剂量服用细辛可能对心功能有一定的抑制作用。

Objective To observe the effects of long term toxicity of Asarum on SD rat＇ s ECG and myocardl enzyme spectrum. Methods SD rats were randomly divided into four erperimental groups： control group, low dose group, middle dose group, and high dose group of Asarum （0.27,0.81,1.35 g/kg）. All rats were garaged once a day , which last 28 days. On the 0^th, 14^th and 28^th day after administrated , the electrocardiogram were texted. The myocardial enzyme were detected after the 28^th gavage. Results compared with the control group, There was no changes in electrocardiogram and myocardial enzyme of the rat in low dose group. In the middle ang high dose group, heart rate decreased significantly. The content of CK, CK - MB, LDH and HBDH increased with significant difference in the medium dose group rats. The content of LDH of high dose group were higher than those in the control group ; the content of serum CK, CK - MB and LDH returned to normal. Conclusion Long - term toxicity of Asarum may has a inhibitory effect on rat＇ s heart function.