摘要
目的建立雪梨膏的含量测定方法。方法采用紫外分光光度法测定雪梨膏中绿原酸类多酚化合物的含量。结果检测波长为329nm,绿原酸在3.5-28.2μg/m L线性关系良好(r=0.9998);平均回收率95.59%;有较好的精密度、重复性及稳定性。结论本方法简便、准确,重现性好。
Objective To establish the determination method of Xueli extract. Method Determine the polyphenol compounds of chlorogenic acids in Xueli extract by UV spectrophotometry. Result The wavelength was 329nm, Chlorogenic acids was linear in the range of 3. 5 -28.2μg/mL（ r=0. 9998 ）, the average recovery was 95.59% , precision, repetition and stability was well . Conclusion The method is simple and accurate, it also has good reproducibility. This method can be applied to determine the Xueli extract.
出处
《湖北中医药大学学报》
2015年第4期37-39,共3页
Journal of Hubei University of Chinese Medicine
基金
2009湖北省教育厅科学技术研究重点项目(2009-091)
关键词
雪梨膏
紫外分光光度法
绿原酸
含量测定
Xueli extract
UV spectrophotometry
chlorogenic acids
determination
