贵州黄牛ANGPTL4基因PCR-RFLP多态性检测

PCR- RFLP Polymorphism of ANGPTL4 Gene in Guizhou Cattle

利用PCR-RFLP技术对关岭黄牛和思南黄牛各46个个体的类血管生成素4(ANGPTL4)进行多态性检测。结果表明,ANGPTL4基因的扩增片段大小为260 bp,经限制性内切酶Mlu CI酶切后,产生AA和AB 2种基因型,没有发现BB基因型,不同基因型经测序发现205T→C突变。2种黄牛品种中均是AA基因型占优势,A等位基因频率均大于B等位基因。卡方检测结果表明2种黄牛品种在该酶切位点处于平衡状态(P>0.05)。

Used PCR - RFLP method to analyze polymorphism of ANGPTIA gene in Guizhou cattle. The results showed that there was one MluCI digestion site in the 260 bp amplified fragment. An T→C mutation at the site 183 bp of this gene by DNA sequencing which produce two sorts of genotypes, AA and AB, however, BB genotype was not founded in this study. The genotype of AA was preponderant in two cattle group, the allele frequency of A were higher than B in all these two breeds. The two breeds were at Hardy - Weinberg equilibrium （ P 〉 0.05 ）.