绿绒海芋Alocasia micholitziana的组织培养及其植株再生的研究

Tissue Culture and Plantlet Regeneration of Alocasia micholitziana

以绿绒海芋Alocasia micholitziana的叶片、叶柄为外殖体,研究不同灭菌剂对外殖体消毒效果的影响、不同激素及浓度配比的培养基对愈伤组织诱导、不定芽分化及增殖的影响。结果表明:(1)消毒处理时叶柄适于用0.1%Hg Cl2浸泡处理8 min,叶片则为7 min;(2)叶柄、叶片在所试培养基中均能诱导出愈伤组织,最佳诱导培养基为MS+2 mg/L 6-BA+2.0 mg/L 2,4-D+0.1 mg/L NAA,叶柄愈伤组织诱导率可达100%;(3)从愈伤组织诱导不定芽的最佳培养基为MS+2 mg/L 6-BA+0.4 mg/L NAA,诱导率可达100%;(4)在培养基MS+2 mg/L 6-BA+0.4 mg/L NAA上,芽增殖效果最佳,苗生长健壮;(5)最佳生根培养基为1/2MS+0.5 mg/L IBA,移栽后苗容易成活;(6)苗移栽时以泥炭土∶珍珠岩∶蛭石(3∶1∶1)成活率较高,可达98%以上,而且小苗生长健壮。

An efficient protocol was established for the regeneration of an ornamental and food crop plant,Alocasia micholitziana using the leaves and petiole explants. The influence of both plant sterilization agents and various growth hormones at different concentrations on callus induction, adventitious bud differentiation and their proliferation were examined. The results revealed that soaking of plant explants in 0.1% of Hg Cl2 solution for 8min was suitable for sterilization. The highest bud induction（100%） was achieved in three plant growth hormone combinations of 6-BA（ 2 mg/L）, 2,4-D（ 2 mg/L） and NAA（ 0.1 mg/L） in the MS culture medium. The optimum concentration for the highest induction（ 100%） of adventitious bud was 2 mg/L of 6-BA with 0.4 mg/L of NAA.The root was successfully induced in the half strength MS medium augmented with 0.5 mg/L of IBA. The in vitro regenerated plants were transf erred to the pot containing peat soil, perlite, and vermiculite（3 ∶ 1 ∶ 1） which showed the maximum survival rate（98%）.