反相高效液相色谱法测定西番莲中的有机酸

Analysis of Organic Acids in Passion Fruit by Reverse Phase High Performance Liquid Chromatography

采用Waters Atlantis C18色谱柱（150 mm×4.6 mm,5μm）,以2%甲醇-98%0.01 mol/L K2HPO4溶液（用H3PO4调节p H2.8）作为流动相,流速为0.6 m L/min,柱温为30℃,进样量为10μL,检测波长为210 nm,建立高效液相色谱同时分析西番莲中10种有机酸的方法。结果表明：该方法相对标准偏差为0.22%-4.00%,平均回收率在88.0%-109.6%,各种酸的线性相关系数r〉0.999 2,检测限为0.001-0.22μg/m L;采用外标法定量。在紫果和黄果西番莲中检测到7种有机酸,其含量趋势呈现为柠檬酸〉L-苹果酸〉L-乳酸〉琥珀酸〉L-抗坏血酸〉酒石酸〉富马酸。该方法具有灵敏度高、测试组分多、速度快等优点,适用于西番莲中多种有机酸的测定。

A method for simultaneo us determination of ten organic acids in passion fruit pulp by reverse phase high performance liquid chromatography was established. Waters Atlantis C18column（150 mm×4.6 mm, 5 μm） was used at 30 ℃ and mobile phase was 2% CH3OH-0.01 mol/L K2HPO4（ p H2.8） with flow rate of 0.6 m L/min. Detection wavelength was at 210 nm. The quantifidation was carried out by external stsndardization method. The relative standard deviations were 0.22%-4.00% and the average recoveries were 88.0%-109.6%. The correlation coefficients for the linear equations were above 0.999 2. and the detection limits were 0.001-0.22 μg/m L. The results indicated that the method was simple, rapid and accurate. The method was applied to the determination of organic acids in passion fruit. Seven organic acids were detected in P. edulis Sims and P. edulis f. flauicarpa Degener, and the trends of organic acids contents were present in citric acid malic acid lactic acid succinic acid ascorbic acid tartaric acidfumaric acid in passion fruit.