摘要
目的:建立叶黄素体外含量测定的方法。方法:本实验通过对叶黄素最大吸收波长的检测、液相色谱条件的筛选、标准曲线的建立、精密度、加样回收率、溶液稳定性等考察建立了叶黄素体外检测方法。结果:样品中的叶黄素浓度(C)与色谱峰面积(A)均呈现良好的线性关系,回归方程的相关系数r为0.9997;叶黄素测定方法的精密度高,重现性好,叶黄素的平均回收率为100.21%,RSD为1.36%;8 h内叶黄素溶液稳定性RSD为0.81%。结论:本方法的精密度、回收率、稳定性和线性范围都达到了药物分析的要求,可用于叶黄素的含量测定。
To establish a method for the determination of lutein in vitro content. Methods： the experiment based on the maximum absorption wavelength of lutein detection, screening of liquid chromatographic conditions, the establishment of standard curve, precision and recovery, solution stability investigation lutein in vitro test method is established. Results： the lutein concentration（C） in the samples and the chromatographic peak area（A） are show A good linear relationship, the regression equation of the correlation coefficient r of 0.9997; lutein determination method of high precision, good reproducibility, lutein the average recovery was 100.21 %, RSD was 1.36 %; Lutein solution stability within 8 h RSD was 0.81 %. Conclusion： the method of precision, recovery, stability and linear range has reached the requirement of pharmaceutical analysis, can be used for the determination of the content of lutein.
出处
《广东化工》
CAS
2015年第15期210-211,共2页
Guangdong Chemical Industry
基金
2014年度大学生创新创业训练计划项目
