人恶性黑色素瘤多发转移裸鼠动物模型的建立

The Establishment of a Model of Multiple Human Malignant Melanoma Metastases in Nude Mice

目的探讨建立人恶性黑色素瘤多发转移裸鼠动物模型的方法,为进一步研究恶性黑色素瘤的发病机制提供动物模型。方法将MV3人黑色素瘤细胞注射于裸鼠背部皮下,待裸鼠背部皮下出现肿瘤后,通过病理学检查及免疫组化技术对动物模型进行鉴定。结果裸鼠皮下接种人黑色素瘤细胞28d后背部皮下相继出现肿瘤,肿瘤组织免疫组化结果显示S-100及HMB45阳性,肿瘤组织HE染色及肺、脾、肝、脑、肾各组织免疫组化结果显示各组织均出现不同程度转移灶,表明成功建立人黑色素瘤多发转移裸鼠模型。结论该研究选择来源于人的恶性黑色素瘤细胞MV3接种于裸鼠背部皮下,28d后成功建立了人黑色素瘤多发转移裸鼠动物模型,该模型为进一步研究黑色素瘤的生物学特性及新药开发提供了实验依据。

Objective To explore the way to establish a model of multiple human malignant melanoma metasta- ses in nude mice so as to provide animal models for further study of the pathogenesis of malignant melanoma. Method Human melanoma cells MV3 were injected into dorsal subcutaneous tissues of nude mice. After tumors appeared in dorsal subcutaneous tissues, the animal models were detected with pathological examina- tion and immunohistochemical techniques. Results 28 days later, nude mice successively had tumors in dorsal subcutaneous tissues after the injection, tumor pathological section immunohistochemistry showed that S-100 and HMB45 were positive, tissue pathological section immunohistochemistry of HE staining, lung, spleen, liver, brain, and kidney showed that all these tissues had metastases to different degrees, which indicated the establishment of a model of multiple human malignant melanoma metastases in nude mice. Conclusion The study chooses human melanoma cells MV3 to inject into dorsal subcutaneous tissues of nude mice and after 28 days, a successful model of multiple human malignant melanoma metastases in nude mice is established. The animal model can provide an experimental evidence for further research of biological characteristics of melano- ma and the development of new drugs.