甘露醇预处理对枣花药愈伤组织诱导的影响

Influence of Mannitol Pretreatment on the Induction of Anther Callus in Ziziphus jujube

以三倍体赞皇大枣和二倍体阜平大枣的花药为试材,以未进行甘露醇处理为对照（CK）,研究了甘露醇预处理浓度（0.1、0.3和0.5 mol/L）和处理时间（2、3和5 d）对枣花药褐化及愈伤组织诱导率的影响。结果表明：甘露醇预处理能够明显促进赞皇大枣和阜平大枣花药愈伤组织的诱导。同一甘露醇浓度预处理下,参试枣品种均以处理2~3 d的褐化率较低,处理3 d的愈伤组织诱导率最高。甘露醇预处理对花药愈伤组织的诱导效果因基因型不同而有所差异,其中阜平大枣的愈伤组织诱导率总体高于赞皇大枣。

The effects of pretreatment concentrations （ O. 1, 0. 3 and 0. 5 mol/L ） and times （ 2, 3 and 5 d ） with mannitol on anther browning and callus induction rate of Ziziphus jujube Zanhuangdazao and Fupingdazao were studied. The results showed that the induction of anther callus of tested varieties could be obviously promoted with mannitol pretreatment. The browning rate was lower and the induction rate of anther callus was highest after treatment 2-3 d under the same pretreatment concentrations of mannitol. The effect of mannitol pretreatment on the induction of anther callus experienced differences with different genotypes, the induction rate of anther callus of Ziziphus jujube Fupingdazao was higher than that of Ziziphus jujube Zanhuangdazao.