猪肉中四环素残留快速检测法的研究

Pork in Tetracycline Residues Rapid Detection Method Research

对猪肉样品的四环素残留量检测的研究。以高效液相色谱法(HPLC)检测猪肉组织中四环素残留,并对这种方法的提取及测定条件等方面进行了探讨。确定最佳提取条件是用5%高氯酸溶液25 m L提取样品中四环素残留,选择最佳测定条件是色谱柱为ODS-C18(6.2 mm×15 mm,5μm),柱温为35℃,进样量为10μL,波长为265 nm,流速为1.0 m L/min。

Based on the high performance liquid chromatography （HPLC） detection of tetracycline residues in pork tissue, and the methods of extraction and determination conditions were discussed.To determine the optimal extraction conditions for 5%perchloric acid solution 25 mL extraction samples tetracycline residues , and the optimal conditions for the determination was a chromatographic column for ODS-C18 （6.2 mm × 15 mm, 5 μm , 35 ℃） , column temperature , amount of sample was 10 μL , 265 nm wavelength , the flow rate of 1.0 mL/min, the experimental detection of pork samples study on the residues of tetracycline.