苦瓜提取物抗氧化及抑制淀粉酶协同作用研究

Study of Synergy Effect of Different Bitter Melon Extracts on Activities of Antioxidant and Inhibition of Amylase through Factorial Design

通过析因设计研究不同溶剂苦瓜提取物的协同作用,为苦瓜功能保健食品的开发提供参考。以邻二氮菲-Fe2+氧化法抗氧化试验及α-淀粉酶的抑制试验研究苦瓜醇提物和水提物的剂量-反应关系,并进行3×3析因设计,通过析因分析研究苦瓜醇提物与水提物的协同作用。结果表明苦瓜的醇提物和水提物在抗氧化及α-淀粉酶抑制作用方面存在协同作用。协同抗氧化效果最好组合是水提物0.2 mg/m L,醇提物0.15 mg/m L;协同抑制α-淀粉酶效果最好的组合是水提物40 mg/m L,醇提物30 mg/m L。

The aim of this experiment was to study the synergies of different solvents extracts derived from bitter melon by factorial design and to provide reference for the development of functional foods bitter gourd. Antioxidant andα-amylase inhibition capacity of the bitter alcohol extract and aqueous extract were detected by phenanthroline-Fe2＋oxidation assay and α-amylase inhibition experiments in this study. Hypoglycemic effect was explored by statistical analysis of factorial design .The results showed that alcohol extract and aqueous extract had synergies effects on antioxidant and α-amylase inhibition activity.Best combination of synergistic antioxidant effect was 0.2 mg/mL aqueous extract, 0.15 mg/mL alcohol extract. The best combination on α-amylase inhibition was 40 mg/mL aqueous extract, 30 mg/mL alcohol extract.