小窝蛋白-1与食管癌细胞迁移之间的关系

Relationship between caveolin-1 and esophageal cancer cells migration in vitro

目的探讨小窝蛋白(Cav)-1在食管癌细胞迁移中的作用,并探讨两者之间的关系。方法体外培养人食管癌TE13细胞株,采用Cav-1小干扰RNA(siRNA)转染TE13细胞(转染组),以未转染细胞为对照(NC)组。采用蛋白免疫印迹(Western blot)实验检测细胞的Cav-1表达水平,采用划痕实验观察细胞迁移情况,探讨Cav-1和食管癌细胞迁移的关系。结果 Western blot实验结果显示,食管癌TE13细胞高表达Cav-1,转染siRNA后细胞Cav-1表达水平较转染前明显下降,转染组和未转染组细胞的灰度值分别为(1.98±0.34)和(3.15±6.10),两者比较差异有统计学意义(t=4.26,P<0.05)。划痕试验结果显示,未转染组在划痕后24 h痕间距明显缩窄,部分痕区域细胞密集;而转染组细胞在划痕后24 h痕间距未见明显缩窄。表明转染后细胞的迁移能力较转染前明显下降。结论 Cav-1在食管癌的发生、发展过程中扮演着促癌基因的角色,抑制Cav-1表达可能抑制食管癌的进展。

Objective To investigate the effect of caveolin -1 （Cav-1） on esophageal cancer cell migration and the rela- tionship between them. Methods Human esophageal carcinoma cell line TEl3 cells were cultured in vitro. The TEl3 cells transfected by Cav-1 small interfering RNA （siRNA） were served as transfection group,and the no-transfection cells were served as control （no-transfeetion group ）. The Cav-1 expression level was detected by protein immunoblotting （Western blot）test,and the cell migration was observed by the scratch test. The relationship between Cav-I and esophageal cancer cell migration was studied. Results The result of Western blot test showed that Car-1 presented high-expression in the e- sophageal carcinoma TEl3 cells,and the Cav-1 expression level after transfecting siRNA decreased significantly compared with pre-transfection. The grey value of transfection group and no - transfection group were 1.98 ± 0.34 and 3.15 ± 6.10, respectively, and there was significant difference between two groups （ t = 4.26, P 〈 0.05 ）. Scratch test showed that in the no-transfection group,the distance between scratch marks got obvious narrow 24 hours after scratching, and the cells were crowded together in partial scratch area, while there was no obvious narrow in the distance between scratch marks 24 hours after scratching in transfection group. These results showed that the migration ability of cells after transfection decreased sig- nificantly compared with pre-transfection. Conclusions Car-1 plays a role of cancer-promoting gene in carcinogenesis and progress of esophageal cancer, and the progress of esophageal cancer might be inhibited by suppressing Cav-1 expression.