泡核桃壳提取物体外抗肿瘤作用研究

In Vitro Anti-tumor Effects of Extracts from Juglans sigillata Shell

目的:研究泡核桃壳提取物对3株人癌细胞SKOV-3、Hep G-2和MGC-803的体外抑制作用。方法:用碱提醇沉法分离提取核桃壳中抗肿瘤活性有效部位,得到3个组分HTKD(D)、HTKE(E)和HTKF(F),应用改良MTT法测定对人癌细胞的抑制作用。结果:在高浓度组分E对3株癌细胞的抑制作用均最明显且呈剂量依赖关系,对3株癌细胞半数抑制浓度(IC50)值也最小。结论:泡核桃壳提取物对体外培养的肿瘤细胞有抑制作用,药效物质主要存在于组分E中。

Objective： To study the anti-tumor effect of Juglans sigiUata shell extracts on three human tumor cell lines including SKOV-3, HepG-2 and MGC-803 in vitro. Methods： Alkali extraction and alcohol precipitation were employed to extract the antitumor activity of effective components HTKD （D）, HTKE （E）and HTKF （F）from Juglans sigillata shell and observe the inhibitory effects with MTr assays. Results： At highest concentration component E could obviously inhibit the growth of three human tumor cells with dose-dependence. In addition, component E inhibited the three tumor cell lines with minimal the median inhibitory concentration （IC50）. Conclusion： The extract of Juglans sigillata shell showed anti-tumor effects and pharmacodynamic material mainly exist in the component E.