血清中结核分枝杆菌IgG/IgM抗体检测在肺结核诊断中的应用价值

Application value of detecting Mycobacterium tuberculosis IgG/IgM antibodies for pulmonary tuberculosis diagnosis

目的评价血清中结核分枝杆菌Ig G/Ig M抗体在肺结核诊断中的应用价值。方法对120例活动性肺结核患者、40例非结核性肺部疾病患者和30例健康献血员的血清标本采用胶体金法进行结核分枝杆菌Ig G/Ig M抗体检测。结果 120例临床诊断的活动性肺结核患者,结核分枝杆菌Ig G/Ig M抗体检测阳性89例(Ig G抗体阳性84例、Ig M抗体阳性5例),阳性率74.2%;40例非结核性肺部疾病患者结核分枝杆菌Ig G/Ig M抗体检测阴性36例,30例健康献血员的结核分枝杆菌Ig G/Ig M抗体检测阴性28例,阴性率91.4%(64/70)。检测菌阳肺结核的敏感性为79.6%(43/54),菌阴肺结核的敏感性为69.7%(46/66)。结论结核分枝杆菌Ig G/Ig M抗体检测对肺结核的诊断具有快速方便、较好的敏感性和特异性。

Objective To evaluate the diagnostic value of detecting tuberculosis Ig G/Ig M antibodies for pulmonary tuberculosis. Methods Mycobacterium tuberculosis Ig G/Ig M antibodies in the serum samples from 120 cases of active pulmonary tuberculosis, 40 cases of non-tuberculosis pulmonary disease and 30 healthy volunteers were detected by colloidal gold technique. Results Eighty-nine of 120 active pulmonary tuberculosis cases were positive of Mycobacterium tuberculosis Ig G/Ig M antibodies（74.2%）, and 36 of 40 patients with other pulmonary diseases and 28 of 30 healthy people were negative results（91.4%）. Conclusion Detection of Mycobacterium tuberculosis Ig G/Ig M antibodies was rapid, convenient, and high sensitivity and specificity in diagnosis of tuberculosis.