过敏性紫癜患者IL-8及IgA/C3水平检测分析

The detection and analysis of serum IL-8 and IgA/C3 in henoch-schonlein purpura

目的通过测定过敏性紫癜患者白细胞介素-8(IL-8)、免疫球蛋白A/补体3(IgA/C3),探讨二者之间的相关性,研究过敏性紫癜患者相应的免疫失衡状况,为临床采用更有效的治疗方法提供理论依据。方法酶联免疫吸附法测定IL-8,全自动生化分析仪测定IgA、C3,并计算IgA/C3。结果双抗体夹心法(ELISA)检测实验组血清中IL-8表达量为(389.00±51.68)ng/L,与对照组相比显著升高(P<0.05)。IgA水平为(306.2±32.21)mg/L,与对照组相比显著升高(P<0.05)。C3水平为(114.5±20.7)mg/L,与对照组比较,差异无统计学意义(P>0.05)。IgA/C3为3.041 0±0.508 5,与对照组相比显著升高(P<0.05)。IL-8与IgA/C3呈正相关关系(r=0.534,P<0.05)。结论过敏性紫癜患者存在细胞因子与免疫球蛋白水平紊乱,体液免疫和细胞免疫均处在高反应状态,IL-8与IgA/C3呈正相关关系。

Objective To investigate the correlation between serum interleukin-8 （IL-8 ）and immunoglobulin/complement 3A （IgA/C3）and immune imbalance in Henoch-Schonlein Purpura（HSP）in children,and provide evidence and guidance for clinical therapy of HSP.Methods Serum IL-8 was measured by Enzyme-linked immunosorbent assay（ELISA）,and IgA and C3 were de-tected by automatic biochemistry analyzer,and calculate the ratio of IgA/C3.Results Serum IL-8 levels were （389±5 1.68）ng/L, significantly higher than that in healthy children （P〈 0.05 ）.Serum IgA levels were（306.2 ± 32.21 ）mg/L,significantly higher （P〈0.05）.Serum C3 levels were （1 14.5 ±20.7）mg/L,of no statistical difference.IgA/C3 were （3.041 ±0.508 5）,significantly higher（P〈0.05）.There was a positive correlation between IL-8 and IgA/C3 （r =0.534,P〈0.05 ）.Conclusion The children of HSP has cytokine and immunoglobulin disturbance,and presents a hyperreactivity in both humoral immunity and cellular immunity. Positive correlation between IL-8 and IgA/C3 was observed.