APPL1在胃癌组织中的表达及意义

Expression of APPL1 in gastric carcinomas and its clinical significance

目的 探讨APPL1在胃癌组织中的表达及意义.方法 选取经手术切除的47例胃癌及23例正常胃组织,采用免疫组织化学SP法和逆转录聚合酶链式反应（RT-PCR）法检测APPL1在胃癌及正常胃组织中的表达情况.结果 APPL1基因的蛋白在胃癌组织中的表达为59.6％ （28/47）,正常胃组织标本组为26.1％ （6/23）;其mRNA在胃癌中相对表达为0.821±0.141,正常胃组织中相对表达为0.731±0.112,两者比较差异均有统计学意义（P＜0.05）.结论 APPL1蛋白和mR-NA在胃癌组织中表达上调,与胃癌的发生及转移有关.

Objective To investigate the expression and significance of adaptor protein containing PH domain,PTB domain,and leucine zipper motif 1 （APPL1） in gastric cancers.Methods Expressions of APPL1 protein and mRNA were detected with immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR） in 47 surgical specimens of gastric carcinomas （GC） and 27 normal gastric tissue specimens,respectively.Results The expression rate of APPL1 protein was 59.6％ （28/47） in GCs,and 26.1％ （6/23） in normal gastric tissues,with a statistically significant difference between two groups （P ＜0.05）.The relative expression of mRNA was 0.821 ±0.141 in GCs,and 0.731 ±0.112 in normal gastric tissues,with a statistically significant difference between two groups （P ＜ 0.05）.Conclusions The expressions of APPL1 protein and mRNA are increased in GCs,with a close relationship between GC and APPL1.