黑蒜提取液对宫颈癌HeLa细胞株的生长抑制作用研究

Study on the inhibitory effect of the black garlic extract on the growth of hela cells

目的 观察黑蒜提取液对宫颈癌HeLa细胞株生长抑制作用,并探讨其可能机制.方法 MTT法测定黑蒜提取液对宫颈癌HeLa细胞株生长抑制率;流式细胞仪检测细胞凋亡及黑蒜提取液作用后肿瘤细胞周期变化;检测B淋巴细胞瘤-2 （Bcl-2）和Bcl-2相关X蛋白（Bax）表达及平均透光度变化.结果 黑蒜提取液对宫颈癌Hela细胞株生长有明显抑制作用,促进细胞凋亡,黑蒜高剂量组凋亡率（53.26±1.78）％,较对照组（3.68±0.11）％差异有统计学意义（P＜0.05）,经黑蒜提取液作用后,宫颈癌HeLa细胞周期改变,其Bcl-2蛋白阳性表达率下降,平均透光度增加;Bax蛋白阳性表达率增加,平均透光度下降.结论 黑蒜提取液可通过调控肿瘤凋亡相关蛋白实现对宫颈癌HeLa细胞株促凋亡和生长抑制作用.

Objective To explore the effect of black garlic extract on HeLa cells and its possible molecular mechanisms.Methods Inhibitory rate of HeLa cells was detected with methyl thiazolyl tetrazolium（MTT）.Flow cytometry annexin V-fluorescein isothiocyanate/propidium iodide （V-FITC/PI） was used to detect tumor cell apoptosis.Flow cytometry was used to measure cell-cycle changes.Immunohistochemistry method was used to detect expressions of tumor proteins bax and Bcl-2.Results Black garlic extract significantly inhibited the growth of HeLa cells.Black garlic extract significantly induced apoptosis of HeLa cells.The apoptosis rate was （53.26 ± 1.78）％ in the black garlic high-dose group,and （3.68 ±0.11）％ in the control group.Black garlic extract affected cell cycle,upregulated bax expression,and downregulated bcl-2 expression.Conclusions Black garlic extract significantly induced the apoptosis of HeLa cells.This effect might be caused through increasing G2/M cells or changing expressions of bax and bcl-2.