福州城市蕃石榴片林地和马尼拉草坪土壤微生物群落结构特征

Characteristics of Soil Microbial Community Structure in Psidium guajava Linn and Zoysia matrella lawns in Fuzhou

采用氯仿熏蒸浸提法和磷脂脂肪酸（phospholipidfattyacid，PLFA）方法，分析了福州城市蕃石榴片林地与其毗邻马尼拉草坪土壤微生物种群数量和群落结构的差异特征。结果表明，0-10cm土层中蕃石榴片林地和马尼拉草坪的微生物生物量碳（MBC）含量分别为289．88和326．89mg·kg^-1；10～20cm土层中蕃石榴片林和马尼拉草坪的MBC含量分别为229．62和269．62mg·kg^-1。蕃石榴片林地与马尼拉草坪的土壤微生物群落结构具有显著差异（P〈0．05）。土层深度对微生物群落结构影响不显著。蕃石榴片林地与马尼拉草坪的各类微生物种类相对丰度差异不大。此外，蕃石榴片林地土壤的革兰氏阳性细菌相对丰度高于草坪，草坪土壤的革兰氏阴性细菌相对丰度高于蕃石榴片林地。研究认为，在城市绿地的建设中，应注意城市片林与城市草坪其自身特有的优势，合理安排城市片林与草坪的布局与种植面积，有利于构建更好更稳定的城市土壤生态系统。

By using dilution plate, fumigation extraction, and phospholipid fatty acid （PLFA） meth- ods, the difference of microbial biomass carbon and microbial community structure between Psidium guajava and adjacent Zoysia matrella lawns were studied. The results showed that soil MBC in Psidium guajava and Zoysia matrella lawns were 289. 88 and 326.89 mg·kg^-1 for 0 - 10 cm depth and 229.62 and 269.62 mg·kg^-1 for 10 -20 cm depth. There were significant differences in microbial community structures between Psidium guajava and Zoysia matrella lawns. And soil depth had no sig- nificant effect on microbial community structure. In addition, relative abundances of the gram positive bacteria in Psidiurn guajava were more than that in Zoysia matrella lawns, and relative abundances ofthe gram negative bacteria in Psidium guajava Linn were less than that in Zoysia matrella lawns. In conclusion, urban forests and lawns have distinctive advantages in urban greenlands construction and reasonable arrangement of layout and acreage of urban forests and lawns is conducive to a better and stable city ecosystem.