抑制素β基因多态性与孕妇子痫前期易感性及新生儿围生结局关联研究

Association of Inhibin Beta Gene Polymorphism and Susceptibility of Pregnant Women and Neonates in Preeclampsia

目的探讨抑制素β基因多态性与孕妇子痫前期易感性及新生儿围生结局关系。方法选取子痫前期孕妇185例,另选同期正常孕妇120例为对照组。采用聚合酶链限制性片段长度多态性(PCR-RFLP)方法对该位点进行基因分型,比较各组基因型频率和等位基因频率的差异。观察两组孕妇妊娠结局。结果抑制素β基因rs505922位点共检出CC、CT和TT等3种基因型。子痫前期组与对照组比较,基因型频率与等位基因频率差异有统计学意义(P<0.05)。子痫前期组内CT和CC基因型新生儿窒息、死亡率均高于TT基因型,差异有统计学意义(P<0.05)。结论抑制素β基因rs505922位点单核苷酸基因多态性与子痫前期密切相关,CT和CC基因型加重了子痫前期的程度,该位点可能是子痫前期孕妇发病的易感位点。

Objective To investigate the association of polymorphism of inhibin beta gene and outcomes of pregnant women in preeclampsia and neonatal perinatal. Methods 185 pregnant women with preeclampsia as subject group and 120 normal pregnant women as control group were involved in this study. The inhibin beta gene in patients and normal controls were detected by polymerase chain restriction fragment length polymorphism（ PCR-RFLP） method. The genotype frequency and allele frequency were analyzed. Results The CC,CT and TT genotypes were detected in inhibin β gene rs505922 locus. There was statistical significance on genotype frequency and allele frequency between two groups（P〈0. 05）. The neonatal asphyxia and mortality rate in preeclampsia pregnant women with CT and CC genotypes were higher than that with TT genotype（P〈0. 05）.Conclusion The polymorphism of inhibin β-rs505922 are closely related with preeclampsia of pregnant women.The inhibin β-rs505922 may be the susceptible site of preeclampsia.