人原发性胃非霍奇金淋巴瘤小鼠皮下移植瘤的病理学特性

Pathological Characteristics of Human Primary Gastric Non-Hodgkin's Lymphoma Subcutaneous Transplantation in Immunodeficient Mice

目的:建立人原发性胃非霍奇金淋巴瘤小鼠皮下移植瘤模型,并探讨其组织病理学特性。方法:取新鲜的人原发性胃非霍奇金淋巴瘤组织移植于NOD/SCID小鼠和BALB/c-nu小鼠左、右肩胛部皮下,观察移植瘤的生长过程;待皮下移植瘤增大,将其取下传代,连续传至6代。同时每代移植瘤组织进行常规病理检测及CD20、CD45RB、Ki67的免疫组织化学检测。结果:在免疫缺陷小鼠体内形成了与人原发瘤形态结构一致的移植瘤,组织中见大量异常增生的大B淋巴细胞。免疫组织化学显示:CD20、CD45RB、Ki67阳性。结论:移植瘤较好地保留了人原发性胃非霍奇金淋巴瘤的组织病理学、生物学特性。

Objective. To investigate the pathological characteristics of human primary gastric nonHodgkin lymphoma subcutaneous transplantation model in immunodeficient mice. Methods. The fresh human primary gastric non-Hodgkin＇s lymphoma tissues were transplated in the subcutaneous position of scapular in NOD/SCID mice and BALB/c-nu mice. When the subcutaneous xenografts grew up, it was passaged continuously for six generations. The growth process of transplanted tumor was observed. H＆E staining and CD20, CD45RB and Ki67 immunohistochemical staining were exploited. Results： Same primary gastric non-Hodgkin lymphoma tumor cells were founded in immunodeficiency mice. A large number of abnormal proliferation of large B-cell lym- phoma were observed and immunohistochemistry identified positive staining of CD20, CD45RB and Ki67. Conclusion： Trasnplantation tumor well preserved primary gastric non-Hodgkin lymphoma＇s characteristics.