具鞘微鞘藻胞外多糖抗紫外辐射活性研究

Research on anti- ultraviolet radiation activity of extracellular polysaccharide from Microcoleus vaginatus Gom.

主要研究具鞘微鞘藻胞外多糖在紫外辐射下对大肠埃希氏菌的保护作用,将大肠埃希氏菌与多糖溶液混合制成菌悬液,再分别进行紫外辐射,培养后计数并计算大肠埃希氏菌紫外存活率及多糖抗紫外辐射率。结果表明:15 W紫外灯照射条件下大肠埃希氏菌存活率略高于20 W紫外灯照射条件下大肠埃希氏菌存活率。20 W紫外灯辐射40 s条件下,400 mg/L的多糖溶液的抗紫外辐射率最高达41%,为200 mg/L、800 mg/L多糖溶液抗辐射率的1.71倍和1.47倍;15 W紫外灯辐射40 s条件下,400 mg/L的多糖溶液的抗紫外辐射率为29%,为200 mg/L、800 mg/L多糖溶液抗辐射率的1.38倍和1.32倍。研究结果表明具鞘微鞘藻胞外多糖对大肠埃希氏菌对抗紫外辐射能力有一定的增强效应,并且400 mg/L浓度多糖溶液的抗辐射性要优于其他浓度。

This paper studied the protective effect of extracellular polysaccharide from Microcoleus vaginatus Gom. for E. coli under UV radiation. In the experiment, we prepared bacterial suspension by mixing E. coli with polysaceharide solution, exposed them to ultraviolet radiation, and cultivated for several days and counted the number of E. coli, then computed UV survival rate of E. coli as well as anti ultraviolet radiation rate of polysaccharide. The results showed that, the UV survival rate of E. coli under 15W UV light irradiation conditions was higher than that under the 20W irradiation. Ir- radiated by 20W UV lamp for 40 seconds, the polysaccharide solution of 400 mg/L can achieved 41% of anti - radiation rate, which was 1.71 times and 1.47 times that of polysaccharide solution of 200mg/L and 800 mg/L respectively. While irradiated by 15W UV lamp irradiation for 40s, the anti ultraviolet radiation rate was 29% for polysaccharide solution of 400 mg/L, which was 1.38 times and 1.32 times that of solution of 200 mg/L and 800 mg/L. The results implied that extracellular polysaccharide from Microcoleus vaginatus Gom. had a certain degree of reinforcing effect for theanti UV radiation of E. coli , and polysaccharide solution of 400 mg/L worked better than that of oth- er concentrations.