摘要
目的探讨不同体外培养方法对小鼠去透明带胚胎发育的影响。方法以胚胎各阶段发育率、囊胚率和囊胚细胞数作为衡量指标,对比微滴单卵法、微滴群卵法、微滴单卵+群卵法、m WOW培养法(the modified"well of well"system)四种体外培养方法 ,探讨不同体外培养方法对去透明带胚胎发育的影响。结果 m WOW培养法、微滴单卵+群卵法、微滴单卵法这三种方法之间的发育率、囊胚率以及囊胚数均有显著差异(P<0.05)。结论m WOW法与其他三种方法相比,更适合作为体外培养去透明带小鼠8-细胞以后阶段的方法 ,以及用于体内移植小鼠胚胎的体外培养方法。
Objective To investigate the effects of different culture methods in vitro mouse zona-free embryonic devel- opment. Methods In various stages of embryonic development rate, blastocyst rate and cell numbers of blastocysts de- veloped were measured as mWOW system （the modified ＂well of well＂ system）, micro-drops single ＋ group, micro- drops single and micro-drops group culture in vitro. The effects of different methods of were compared in vitro embryo development zona-free. Results Embryonic development rate, blastocyst rate and cell numbers of blastocysts developed were significantly higher in mWOW system culture than micro-drops single ＋ group, micro-drops single and micro- drops group culture. Conclusion Compared with micro-drops single ＋ group, micro-drops single and micro-drops group culture, mWOW system method is more suitable for zona-free cultured in vitro mouse embryos.
出处
《中国现代医生》
2015年第16期18-20,共3页
China Modern Doctor
基金
辽宁省科技厅科学计划项目(2011408004)
关键词
胚胎培养技术
胚胎发育
mWOW法
Embryo culture techniques
Embryonic development
mWOW method
