半制备液相色谱法分离制备内生真菌次级代谢产物条件优化

Optimization of onditions of Separation and Preparation of Endophytic Fungi Secondary Metabolites by Semi-Preparative Liquid Chromatography

为了提高物质的制备量和分离度,以流动相比例作为主要考察对象,在分析液相色谱法分离检测结果的基础上,采用半制备液相色谱法对内生真菌次级代谢产物的制备条件进行了优化实验。最终确定梯度洗脱程序为：0-10 min,流动相（水：甲醇）体积比：90∶10;15-50 min,流动相（水：甲醇）体积比：85∶15;50-60 min,流动相（水：甲醇）体积比：20∶80。

In order to improve preparation quantity and separation degree, proportion of mobile phase was stud-ied as the main object, the preparation condition of endophytic fungus secondary metabolites was optimized for semi-preparation HPLC based on the analyzing results of HPLC. The ideal gradient elution program was as follows：first mobile phase at the ration of water and methanol was 90∶10 for 0 to 10 min, second mobile phase at the ration of water and methanol was 85∶15 for 15 to 50 min, and the last mobile phase ration of water and methanol was 20∶80 for 50 to 60 min.