HCG治疗子宫内膜异位症大鼠模型的超微结构变化及意义

Ultrastructural changes and significance of endometriotic rat model with HCG treatment

目的:探讨人绒毛膜促性腺激素(HCG)治疗子宫内膜异位症大鼠的疗效及机制。方法:诱导法成功建立子宫内膜异位症大鼠模型24只,随机分为4组,每组6只,分别以每天19.4 IU/100 g(HCG低剂量组)、25.8 IU/100 g(HCG中剂量组)和51.6 IU/100 g(HCG高剂量组)的HCG治疗15 d,并以生理盐水作为对照,比较各组治疗前后异位病灶体积的变化及异位内膜、在位内膜电镜下超微结构的改变。结果:HCG治疗后,HCG低、中和高剂量组的异位病灶体积均较治疗前减小(P<0.05);治疗前子宫内膜和异位内膜胞浆内可见大量线粒体、内质网及核糖体;治疗后部分细胞结构不清,部分线粒体嵴减少或消失,细胞致密、萎缩,胞浆中自噬体增多,线粒体和内质网肿胀。结论:HCG可能使在位和异位内膜中的细胞功能代谢减退,从而对子宫内膜异位症大鼠有治疗作用。

AIM： To investigate whether and how human chorionic gonadotropin（ HCG） treatment ameliorates endometriosis in the endometriotic rat model. METHODS： The rat model of endometriosis was established and the model rats were divided into 4 groups. The rats in HCG groups were treated with 19. 4,25. 8 and 51. 6 IU /100 g of HCG every day（ low-dose HCG,medium-dose HCG and high-dose HCG,respectively）. The rats in control group were treated with0. 9% Na Cl. After 15 days（ 3 estrous cycles）,the ectopic lesion volume and ultrastructural characteristics in eutopic and ectopic endometria were investigated. RESULTS： After HCG treatment,the volume of endometriotic lesions was significantly smaller than that before treatment. Numerous and mitochondrial,endoplasmic reticulum and ribosomes were observed in the cytoplasm of eutopic and ectopic endometrium before treatment. After treatment,some cell structures were not clear,and mitochondrial cristae decreased or disappeared partly. Some cells were densed and shrinkage,autophagosome in cytoplasm increased,and mitochondria and endoplasmic reticulum swelt. CONCLUSION： HCG therapy appears to be an effective treatment for endometriosis in rats attributed to its influence on cell metabolism dysfunction of eutopic and ectopic endometria.