C-型咽侧体抑制素(AST-C)和促前胸腺激素释放激素(PTTH)基因在家蚕脑组织中的时空表达分析

Temporal and spatial expression analysis of allatostatin C(AST-C) and prothoracicotropic hormone(PTTH) genes in the brain of the silkworm,Bombyx mori(Lepidoptera: Bombycidae)

【目的】咽侧体抑制素在昆虫体内具有重要的调控功能。本研究比较了家蚕Bombyx mori C-型咽侧体抑制素(allatostatin C,AST-C)与促前胸腺激素释放激素(prothoracicotropic hormone,PTTH)基因在不同发育阶段家蚕脑组织中转录表达的模式及其在脑组织的表达定位,以期为家蚕AST-C的功能研究提供重要的线索。【方法】利用脑组织芯片数据分析比较AST-C和PTTH基因在家蚕脑组织中的发育表达特征,RT-PCR验证其芯片数据,分析AST-C在不同发育阶段家蚕中枢神经系统中的表达模式,并用全组织包埋原位杂交技术对AST-C和PTTH在脑组织的表达进行定位。【结果】AST-C和PTTH在不同发育阶段家蚕脑组织中有相似的转录表达模式,且都在脑组织外侧一对神经分泌细胞中表达。【结论】AST-C可能与PTTH以相同的转录表达模式共同参与家蚕的变态发育调控。

[ Aim ] Allatostatins play important regulatory roles in insects. The transcription expression patterns of allatostatin-C （AST-C） and prothoraeicotropie hormone （FFFH） genes in different developmental stages of Bombyx mori were compared, detected to supply important clues for the functional and their expression location in the brain was study of AST-C gene in B. mori. [ Methods ] Microarray data were analyzed to compare the developmental expression patterns of AST-C and PTTH genes. RT-PCR was conducted to corroborate the microarray data and analyze the developmental expression pattern of AST-C in the central nervous system. The transcription location of AST-C and PTTH genes in the brain was observed using whole mount in situ hybridization. [ Results ] The results showed that AST-C and PTYH genes shared the similar transcription pattern, and both were expressed in a pair of laterally neurosecretory cells of the brain of the silkworm. [ Conclusion] AST-C and FFFH genes may be involved in the regulation of metamorphosis through the same transcription expression pattern.