田间系统调查表明山东省农区烟粉虱优势种为Q隐种

Widespread displacement of the exotic whitefly species Bemisia tabaci B by Bemisia tabaci Q in fields in Shandong,China

【目的】为了揭示山东省农区烟粉虱Bemisia tabaci隐种的分布情况,对2013年7-8月份期间在该省不同农区采集的烟粉虱隐种组成进行了系统调查研究。【方法】在山东省15市22个地点作物上采集了69份烟粉虱样品,利用mt COI基因PCR-RFLP方法进行了分子鉴定,并分析了烟粉虱Q隐种在不同寄主作物及地理区域内的分布状况。【结果】22个采集地点中,17个地点的采集样品全部为烟粉虱Q隐种;其他5个地点(潍坊、菏泽、泰安、淄博和临沂)的烟粉虱Q隐种所占比例均大于90%,B隐种比例较低(<10%)。烟粉虱Q隐种比例在5种寄主(茄子、辣椒、番茄、黄瓜和棉花)种群间以及在山东省东部丘陵、中部山地和西部平原地区间均没有显著差异。【结论】2013年田间调查发现烟粉虱Q隐种在山东各地已广泛取代B隐种成为该地区优势种群。

[Aim] To reveal the distribution and infestation status of Bemisia tabaci cryptic species, a systematic and comprehensive survey on its species composition was conducted in Shandong, China. [Methods] Sixty-nine collections of adult whiteflies were sampled from 22 locations distributed in 15 counties in Shandong during July - August, 2013, the cryptic species of the 1 387 individuals was determined based on mitochondrial cytochrome oxidase I （mtCOI） PCR-RFLP method, and the percentages of B. tabaci Q on different host plants and in geographical regions were analyzed. [ Results ] The results showed that the percentage of B. tabaci Q was 100% in 17 locations and was 〉90% in five locations （ Weifang, Heze, Tai＇ an, Zibo, and Linyi）, where B. tabaci B was found with low percentage （ 〈 10% ）. The average percentages of B. tabaci Q did not differ significantly among the populations on the five hosts （eggplant, pepper, cucumber, tomato and cotton） and in the three areas （the western plain region, the eastern hilly region and the central mountains region ）. [ Conclusion ] The results indicate that B. tabaci Q is predominant in all the investigated locations, suggesting that B. tabaci Q has completely displaced B. tabaci B in most regions of Shandong, China.