盐酸阿霉素与人端粒DNA相互作用的电化学研究

Investigation on Interaction of Doxorubicin Hydrochloride with Human Telomere DNA by Electrochemical Methods

建立了石墨烯修饰玻碳电极上研究盐酸阿霉素（DOX）与人端粒DNA相互作用的电化学方法。实验发现,在p H 6.0 PBS缓冲溶液中,DOX在-0.585 V和-0.638 V处有1对氧化还原峰,氧化峰电流与DOX的浓度在0.03-0.8μmol/L和0.8-10μmol/L范围内分段呈良好的线性关系,检出限（S/N=3）为0.01μmol/L。对0.5μmol/L的DOX进行11次平行测定,相对标准偏差为3.5%。将不同浓度的人端粒DNA加入DOX,DOX的氧化峰电流明显降低,且紫外光谱有蓝移增色效应,表明二者发生了相互作用,结合比为1∶1,结合常数为1.11×10^3L/mol。

Electrochemical behavior of the anticancer doxorubicin hydrochloride （DOX）and the interaction of DOX with human telomere DNA were investigated by cyclic voltammetry in aqueous medium using pH 6. 0 PBS buffer. The results showed that a pair of reversible oxidation - reduction peaks were observed at -0. 585 V and -0. 638 V in pH 6. 0 PBS buffer. There existed excellent linearities between oxidation peak current and concentration of DOX in the ranges of 0. 03 - 0. 8 μmol/L and 0. 8 - 10 μmol/L by linear sweep voltammetric method. Detection limit（ S/N = 3 ） was 0. 01 μmol/ L. The relative standard deviations of the oxidation peak current obtained from 11 determinations of the same solutions containing 0. 5 μmol/L DOX were 3.5%. The oxidation peak current of DOX gradually decreased with the adding of various concentrations of human telomere DNA, due to interaction of DOX with human telomere DNA. The binding ratio was calculated to be 1 ： 1 and the binding equilibrium constant was 1.11 × 10^3 L/mol.