摘要
目的探讨少精子症与精子形态之间的相关性。方法选取少精子症组135例、正常精子数量组87例、健康对照组56例,采用改良巴氏染色法用西班牙计算机辅助精子分析(CASA)全自动精子质量分析系统,按世界卫生组织(WHO)《人类精液检查与处理实验室手册(第5版)》精子形态学分类标准进行精子形态学分析。结果 135例少精子症组中检出畸形精子症106例,阳性率为78.5%;87例正常精子数量组中检出畸形精子症27例,阳性率31.0%;56例健康对照组中检出畸形精子症16例,阳性率为28.6%,少精子症组的阳性率明显高于与其他2组。少精子症组中的正常形态精子,异常形态精子,小头精子,异常顶体精子,角度异常,尾部异常,胞浆小滴与其他2组比较,差异有统计学意义(P<0.05);正常精子数量组与健康对照组比较,差异无统计学意义(P>0.05)。结论少精子症的异常精子形态明显高于正常精子数量组和健康对照组,异常精子形态以头部异常为主。
Objective To investigate the correlation between oligospermatism and sperm morphology. Methods 135 cases of oligospermatism,87 men with normal sperm count and 56 healthy men as the control group were selected and performed the sperm morphology analysis by adopting the modified Papanicolaou staining and the computer-aided sperm analysis (CASA) full automatic sperm quality analyzer according to the sperm morphology classification standards in Human Semen Examination and Processing Laboratory Manual(fifth version). Results In 135 cases of oligospermatism group, 106 cases of teratozoospermia were detected with the positive rate of 78.5 % ;in 87 cases of normal sperm count group,27 cases of teratozoospermia were detected with the positive rate of 31.0% ;in 56 cases of the healthy control group, 16 cases of teratozoospermia were detected with the positive rate of 28.6 %, the positive rate in the oligospermatism group was significantly higher than that in the other two groups. Compared with the other two groups,the oligospermatism group has statistically significant difference in the oligospermatism group had statistically differences; while the normal sperm count had no statistically significant difference compared with other two groups. Conclusion The positive rate of abnormal sperm morphology in oligospermatism is obviously high- er than that in the normal sperm count group and healthy control group,the abnormal sperm morphology is domina- ted by the sperm head abnormality.
出处
《检验医学与临床》
CAS
2015年第16期2408-2409,共2页
Laboratory Medicine and Clinic