2013-2014年九江地区献血者血液核酸筛查结果分析

Analysis of screening results by nucleic acid amplification testing in volunteer blood donors in Jiujiang,2013- 2014

目的调查研究九江地区献血人群中核酸检测的结果,以探讨核酸检测的必要性,完善血液安全措施。方法 2013年3月-2014年12月采集九江地区无偿献血标本共80 163份,用ELISA试剂进行HBs Ag、抗-HCV、抗-HIV和抗-TP的2次检测,用速率法进行ALT的2次检测,上述检测合格的标本和ELISA检测单试剂不合格的标本共78 061份,进行核酸检测。结果 78 061份标本经核酸检测,有154份为拆分阳性,阳性检出率为0.197%(154/78 061);经鉴别试验有104份为HBV阳性,阳性率为67.53%(104/154),其中11份为HBs Ag单试剂不合格。48份HBV DAN阳性的两对半检测,有79.17%抗-HBc阳性。结论即使是采用灵敏度再高的HBs Ag酶免检测试剂,也无法检出HBV隐匿性感染,而核酸检测正好可以弥补其中的不足,因此开展核酸检测可以更好地保障血液安全。

Objective To research the necessity of the nucleic acid test and to improve blood safety measures through investigating the results of the nucleic acid test of Jiujiang district. Methods From March 2013 to December 2014, 80 163 blood samples were collected from the donors. Then, the levels of HBsAg, anti-HCV, anti-HIV and anti-TP were detected by two rounds of ELISA, and the level of ALT was quantified by two rounds of velocity method. 78 061 blood samples, whether or not they were tested reactive by one reagent of ELISA, would be tested by nucleic acid test. Results 154 blood samples out of 78 061 were positive at a detection rate of 0. 197% （154/78 061 ）. From the identification test, 104 blood samples were HBV positive at a rate of 67.53% （104/154）. Among these infected samples, 11 blood samples were tested HBsAg positive by one reagent. 48 HBV-DNA positive samples were detected with two half and half and 79. 17% were anti-HBe positive. Conclusion Even though the highly sensitive HBsAg-ELISA reagent is diflqeult to confirm the HBV occult infection, but the addition of a nucleic acid test improve the quality of detection and thus, ensures the safety of blood for clinical use.