模拟红细胞电子交叉配血的回顾性分析

Retrospective analysis of the simulated application of electronic blood cross matching tests

目的通过红细胞输注的回顾性分析,探讨电子交叉配血的影响因素。方法采用全自动血型分析仪IH-1000检测我院受血者与供血者的ABO血型、不规则抗体筛查以及直接抗人球蛋白试验(以下简称直抗试验),交叉配血同时采用电子交叉配血和微柱凝胶法配血。结果在对红细胞进行电子交叉配血发现,14 762例受血者中有3例2次血型结果不一致,占0.02%,有101例不规则抗体筛查阳性,占0.68%,有26例交叉配血不合,占0.17%;29 608 U RBC供血者中有26单位RBC直接抗球蛋白试验阳性,占0.09%,出现次侧交叉配血不合。结论供血者直抗试验阳性不能进行电子交叉配血。只有具备ABO/Rh D2次血型鉴定准确、不规则抗体筛查阴性、供者直接抗人球蛋白阴性的结果适合电子交叉配血。

Objective To analyze the affecting factors of electronic blood cross matching test in clinical application. Methods Automatic blood analyzer IH-1 000 was used to detect the hospital＇s recipient and donor ABO blood groups and to conduct irregular antibody screening, erossmatch and direct Coombs test. Results In erythrocyte electronic crossmateh, among 14 762 cases of recipients, three were found to contain two inconsistent results, aceonnting for 0. 02%. There were 101 cases of irregular antibody screening positive, accounting for 0. 68%. There were 26 cases of cross-matching that had issues of blood incompatibility, accounting for 0. 17%. Among 29 608 units from blood donors, 26 RBC RBC units were positive in direct Coombs test, accounting for 0.09% and resulting in incompatibility for cross-match. Conclusion The results have identified the accuracy of the ABO blood group. Only those patients tested negative in irregular antibody screening and direct Coombs should be permitted for electronic crossmatch.